How can cell migration or invasion assays be measured on a microplate reader?
For instance, tumor cells undergo a transformation where they metastasize to another area of the body by invading different tissues. Or leukocytes need to migrate to different areas of the body during infection. Therefore, assays that can measure cell migration and cell invasion are important tools to have for a microplate reader.
One way to measure cell invasion is to use the FluoroBlokTM assay from BD. Well chambers with a membrane are placed into a 96 well microplate and fluorescently labeled cells are seeded on top of the membrane. Measurements are taken from the microplate bottom to measure how quickly the fluorescently labeled cells invade through the membrane. Review this application note to learn more: Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok™ and FLUOstar OPTIMA.
Another way to measure cell migration is to use the Platypus® OrisTM Cell Migration Assay. Here a plug is placed in the center of a microplate well and fluorescently labeled cells are seeded on the outside of the plug. The plug is the removed and measurements are taken to watch how quickly the migration of the cells occurs toward the middle of the well. Review this application note to learn more: Analysis of migration using the Oris(TM) Cell Migration Assay-TriCoated kit on the POLARstar Omega.