Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Assessing parallelism using Parallel Line Analysis (PLA) in MARSMario Schneider (1), Michael Haitz (1), Klaus-Jürgen Ziegler (1), Carl Peters (2), (1) BMG LABTECH GmbH, Ortenberg, Germany; (2) BMG LABTECH Inc., Cary NC, USA, 02/2019
Development of a rapid HTRF insulin assayZ.J. Farino (1) , T.J. Morgenstern (1) , J. Vallaghe (2) , N. Gregor (2) , P. Donthamsetti (1) , P.E. Harris (1) , N. Pierre (2) , R. Freyberg (3) , F. Charrier-Savournin (2) , J.A. Javitch (1) , Z. Freyberg (1), (1) Columbia University , (2) Cisbio , (3) Yeshiva University, 04/2016
Fluorescence polarization based assay suitable for screening H-Prostaglandin D Synthase inhibitorsCarl Peters, BMG LABTECH, 07/2015
Using SPARCL technology to develop immunoassays for biomarker detection and pharmacokinetic studiesWenhua Xie (1) , Mark Cameron (1) , Carl Peters (2), (1) Lumigen , (2) BMG LABTECH, 06/2015
Cisbio's HTRF cortisol assay performed on the PHERAstar FSJean-Luc Tardieu , Francois Degorce, Cisbio, 03/2007
Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in miceRead article
ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet α Cell HeterogeneityRead article
The PARP inhibitor, olaparib, depletes the ovarian reserve in mice: implications for fertility preservationRead article
Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise statusRead article
Am. J. Physiol. Endocrinol. Metab.
The day-to-day reliability of peak fat oxidation and FATMAXRead article
Eur. J. Appl. Physiol.