High throughput applications with the SPECTROstar Nano
The imbalance in the brain of some transition metal ions (iron, copper, manganese) is thought to be involved in the progression of some neurodegenerative diseases, although a precise mechanism of action has not been established. This laboratory researches redox-active plasmatic iron and it wants to investigate novel chelators that could decrease metal reservoirs within cells and blood-brain barrier models. Since most metal-chelating compounds are chromophoric, the UV-Vis absorbance ability of the SPECTROstar Nano can be used to find high affinity chelators that may be used to restore metalloneurochemical balance. A high-throughput, competition assay is proposed with iron- or copper-chromophore ligands and then cell toxicity will be tested via the MTT assay on possible therapeutic chelators.
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