The Importance of studying cells cultured in vitro under physiological conditions

juin 21, 2019

Whether studying a particular disease or a particular tissue the relevance of the data obtained from cell-based assays are always going to be affected by how closely the conditions used in the study recapitulate the physiological environment in vivo.

Image of Prof Dr Giovanni Mann
Prof Dr Giovanni Mann
Prof. Vascular Physiology
King's College London

Important and relevant parameters include temperature, humidity, CO2 and O2, which differ depending on the location of the tissue and its access to blood supply. Fortunately, these are all parameters that can now be measured and controlled in the laboratory for in vitro cell culture.


Historically, primary cells and transformed cell lines were cultured in 5% CO2 incubators at 37ºC, 85-90 % humidity and gassed with room air irrespective of the origin of the cells. In the human body, cells are exposed to 37ºC and notably at lower temperatures metabolism reduces rapidly, thus there is a requirement for studies to be conducted under stable, physiological temperature. CO2 tension of 5% is used to maintain the pH in cell culture growth media around pH 7.4, noting that acidification of the media at lower CO2 tension quickly leads to loss of cell viability. Primary cells and cell lines are maintained in media rich nutrients, with a high humidity 85-90 % limiting evaporation from the media and thereby obviating loss of volume that could increase the concentration of salts in the media to potentially lethal levels.


Maintaining cell viability ensures that cells can be studied over long term periods but what if the tissue from where the cells were derived are not normally exposed to atmospheric oxygen? Cells very rarely experience atmospheric oxygen levels, and thus it is questionable whether data generated from cells exposed to atmospheric oxygen levels accurately reflect reactions occurring in tissues in the body? Atmospheric O2 is 21.1% at sea level and O2 levels in the body notably vary significantly depending on the organ/tissue of interest. The table below reproduced from Carreau et al.1 provides an overview of the differences in physiological oxygen levels experienced by different tissues.

Normal values of oxygen in various human tissues:    
Tissue mmHg % O2
Air 160 21.1
Trachea 150 19.7
Alveoli 110 14.5
Arterial Blood 100 13.2
Venous Blood 40 5.3
Cell 9.9-19 1.3-2.5
Mitochondria <9.9 <1.3
Brain 33.8+/-2.6 4.4+/-0.3
Lung 42.8 5.6
Skin(sub-papillary plexus) 35.2+/-8 4.6+/-1.1
Skin(dermal-papillae) 24+/-6.4 3.2+/-0.8
Skin(superficial region) 8+/-3.2 1.1+/-0.4
Intestinal Tissue 57.6+/-2.3 7.6+/-0.3
Liver 40.6+/-5.4 5.4+/-0.7
Kidney 72+/-20 9.5+/-2.6
Muscle 29.2+/-1.8 3.8+/-0.2
Bone Marrow 48.9+/-4.5 6.4+/-0.6

Clearly when performing assays maintaining all cells at atmospheric oxygen tension does not reflect physiological conditions, which has been recognised and addressed by a numbers of companies manufacturing incubators and workstations.


Workstations from companies like Baker-Ruskinn produce workstations providing the ability to adapt cells and perform usual laboratory bench tasks such as cell passage, cell microscopy under closely regulated O2 and CO2 conditions. While incubator manufacturers are introducing not only CO2 regulated incubators but O2 and CO2 controlled incubators to simulate physiological conditions. BMG LABTECH provides the perfect microplate reader in the CLARIOstar Plus with Atmospheric Control to perform either short end point readings or long term kinetic studies over 72 hours while also maintaining CO2 and O2 tension together with 37ºC.


In their recent Physiological Reviews article2 Keeley and Mann provide a comprehensive narrative providing a framework for researchers to follow for culture of cells in vitro under conditions relevant to an organ or tissue. This comprehensive review also highlights multiple publications describing the changes observed in all tissue types including endothelial cells, circulating cells, intestinal cells, bone marrow-derived stem cells when studied under normoxic (physiological oxygen) conditions. The review concludes with a series of guidelines that outline the process for studying tissue culture under defined physiological normoxia in vitro including:

 

  • The use of appropriate conditions, especially the physiological oxygen tension
  • Pre-equilibration of culture media solutions and plasticware to the correct oxygen tension before commencing
  • Control of cell/tissue oxygenation
  • O2 permeable plasticware
  • Optimum surface area to volume ratio
  • Increasing ambient oxygen in a designataed workstation to compensate for oxygen consumption by metabolically active cells

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Thus study of cell cultures under their physiological conditions should provide more relevant phenotypic data that extrapolates to animal models and beyond. This could ultimately provide insights into disease pathology, pharmacodynamics and increase the speed and efficiency of drug development.

References

 

1.    Aude Carreau,  Bouchra El Hafny-Rahbi, Agata Matejuk, Catherine Grillon, and Claudine Kiedaa. Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.    J Cell Mol Med. 2011 Jun; 15(6): 1239–1253. (Published online 2011 May 29. doi: 10.1111/j.1582-4934.2011.01258.)


2.    Keeley TP, Mann GE. Defining physiological normoxia for improved translation of cell physiology to animal models and man. Physiol. Reviews 99:161-234 (doi: 10.1152/physrev.00041.2017).

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