NGS sample preparation laboratories such as sequencing facilities are conducting nucleic acid quantification assays on a daily basis but technology that provides adequate throughput and precision remains a challenge. Here we show that combination of Qubit assays with the FLUOstar Omega® multi-mode microplate reader is ideally suited to measure a wide range of nucleic acids and improves compared to the single tube fluorometer on sensitivity, precision and speed. DNA as well as RNA was quantified using the high-throughput equipment and showed comparable results as with standard quantification method. Especially large laboratories that perform automated high throughput sample preparation will profit from this technology.
Streamlining Next Generation Sequencing quality management using high throughput fluorometric DNA/RNA quantification
Ulrike Bönisch, Laura Arrigoni, Diana Santacruz, Nadia Kress Deep Sequencing Facility, Max Planck Institute of Immunobiology and Epigenetics Freiburg 09/2018