Path length correction
For many assays such as DNA quantification and protein determination, users frequently compare absorbance results from a microplate reader with those from a 1 cm path length cuvette spectrophotometer.
All microplate readers from BMG LABTECH that are capable of reading absorbance have a 1 cm path length correction built in via an internal fitting algorithm. Using this correction technique, the 1 cm data from a microplate assay is given with no calculations or estimations made by the user. The path length correction feature can be turned on or off in the software as your needs dictate.
A linear regression comparison of data from a BMG LABTECH microplate reader and 1 cm cuvette spectrophotometer produces an r² value of over 0.99 in the UV and visible portions of the spectrum.