Highly sensitive NanoBRET measurements using the LVF monochromator or filter-based readers
With the NanoLuc® luciferase, Promega has created the next generation of BRET assays. The NanoLuc® enzyme is smaller in size than other luciferases. At the same time its emission is brighter by several factors. These properties make the NanoLuc® the perfect BRET donor. As BRET acceptor, a fluorescently labeled fusion protein are used such as the HaloTag® that emits light in the red range (>610 nm).
The NanoBRET assay technology is intended for studying protein-protein interactions in living cells. The proteins of major interest are membrane receptors such as G-protein coupled receptors (GPCR) and receptor tyrosine kinases (RTK).
All BMG LABTECH microplate readers can be equipped with optimized filters to record the two BRET signals coming from NanoLuc® luciferase and fluorophore.
The CLARIOstar® with its linear variable filter (LVF) monochromator™ matches the two NanoBRET wavelengths without special filters. The flexibility to use the LVF monochromator for NanoBRET™ measurements enables discrimination of donor luminescence and two different acceptor fluorophores. This features a novel receptor internalization assay.
The boxes below lead on to NanoBRET related products and application notes.