février 08, 2022
Binding Assays - More Than One Way to Achieve your Goals
For many years, binding assays have been used extensively for fundamental biological research and are a key part of pharmacology and medicinal chemistry. Affinity binding assays are typically used to determine the strength of binding of a ligand (protein, peptide, or small molecule drug) to a target biomolecule. Therefore, many experiments are aimed at determining the binding affinity of potential binding partners represented by the equilibrium dissociation constant KD, the concentration of ligand at which half the binding sites on a biomolecule are occupied at equilibrium. The target biomolecule could be another protein, antibody, DNA, RNA, G-protein coupled receptors, kinases, or a multitude of other receptors, which when activated by a ligand, produce a pharmacological effect in the body.
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