diciembre 06, 2019
Targeting a kinase? Find out how BMG LABTECH microplate readers measure interaction with kinases, kinase activities and kinase inhibitors in high throughput.
Screening
Drugs acting on a therapeutic target have to be identified out of libraries of thousands to millions of compounds. Due to the large amount of tests necessary for a screen, they are mainly conducted in 384 and 1536 well plates to minimize reaction volumes per sample.
Speed and sensitivity are key requirements for high-throughput screening microplate readers. A 1536 well microplate needs to be measured in seconds, maximally a few minutes. Furthermore, the fast measurement of 10 µl or even lower volume samples need to be very precise and stable to clearly identify hits from a vast number of compounds. In addition, the reader needs to be reliable, as errors occurring during a screen result in repetitions, loss of time, reagents and consequently money. The PHERAstar FSX masters all these requirements in all detection modes, making it popular in screening facilities.
For screening assay development and testing, it is not necessary to block the dedicated screening reader, the CLARIOstar Plus is the perfect addition to develop and characterize an assay before transitioning to high throughput.
Resources
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
- 348
Binding kinetics: high throughput assay for kinase inhibitors
Krumm A (1) , Georgi V (2) , Schiele F (2) , Fernández-Montalván A (2), (1) BMG LABTECH GmbH , (2) Bayer AG, Drug Discovery, Pharmaceuticals, Berlin, Germany, 06/2020 - 342
Dual Channel Kinetic assays for detecting ligand bias at GPCRs
Paul Tewson (1) , Scott Martinka (1) , Kevin M. Harlen (1) , Thom Hughes (1) , Anne Marie Quinn (1) , Sam R.J. Hoare (2) , Carl Peters (3), (1) Montana Molecular, Bozeman, MT , (2) Pharmechanics, Owego, NY , (3) BMG LABTECH Inc., NC, USA, 11/2019 - 335
Analyze binding kinetics with HTRF
Nicolas Pierre , Thomas Roux, Cisbio Bioassays, Codolet, France, 05/2019 - 317
NanoBRET™ assay quantitatively evaluates VEGF binding to the VEGFR2 in real-time in living cells
Kilpatrick LE (1) , Friedman-Ohana R (2) , Alcobia DC (1) , Riching K (2) , Peach CJ (1) , Wheal AJ (1) , Briddon SJ (1) , Robers MB (2) , Zimmerman K (2) , Machleidt T (2) , Wood KV (2) , Woolard J (1) , Hill SJ (1), (1) Division of Physiology, Pharmacology and Neuroscience, University of Nottingham, UK , (2) Promega Corporation, Madison, WI, USA, 01/2018 - 316
CRISPR/Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking
Carl White (1,2) , Ethan See (1,2) , Kevin Pfleger (1,2,3), (1) Molecular Endocrinology and Pharmacology, Harry Perkins Institute of Medical Research, Australia , (2) Centre for Medical Research, The University of Western Australia, Australia , (3) Dimerix Limited, Nedlands, Australia, 01/2018
abril 18, 2019
Monoclonal antibody drugs – a history of success and a bright future!
abril 10, 2019
High-throughput screening (HTS)
enero 21, 2019
Lab Automation – Pipetting many thousands of samples by hand is awful
Binding pathway determines norepinephrine selectivity for the human β1AR over β2AR
Read articleCell Res
Discovery and Characterization of BAY 1214784, an Orally Available Spiroindoline Derivative Acting as a Potent and Selective Antagonist of the Human Gonadotropin-Releasing Hormone Receptor as Proven in a First-In-Human Study in Postmenopausal Women
Read articleJ Med Chem
Structure of the human gonadotropin-releasing hormone receptor GnRH1R reveals an unusual ligand binding mode
Read articleNat Commun
The Sensing Liver: Localization and Ligands for Hepatic Murine Olfactory and Taste Receptors
Read articleFront Physiol
Decreased striatal adenosine A2A-dopamine D2 receptor heteromerization in schizophrenia
Read articleNeuropsychopharmacology
