Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Studying the molecular mechanism of viral replication in real time using the CLARIOstar Plus with ACUMarko Noerenberg (1) , Vincenzo Ruscica (2) , Alfredo Castello (1,2*), (1) MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland (UK) , (2) Department of Biochemistry, University of Oxford, South Parks road, OX1 3QU, Oxford, (UK) *Correspondence to: firstname.lastname@example.org, 11/2020
Binding kinetics: high throughput assay for kinase inhibitorsKrumm A (1) , Georgi V (2) , Schiele F (2) , Fernández-Montalván A (2), (1) BMG LABTECH GmbH , (2) Bayer AG, Drug Discovery, Pharmaceuticals, Berlin, Germany, 06/2020
Ubiquitination monitoring in real-time: the ﬂuorescence polarization-based method UbiRealTyler G. Franklin , Jonathan N. Pruneda, Oregon Health & Science University, Dept. of Molecular Microbiology and Immunology, Portland, OR, USA, 05/2020
Elucidating PROTAC MoA with live cell kinetic monitoring of ternary complex formation and target protein ubiquitinationKristin Riching , Amy Landreman , Danette Daniels, Promega, Madison, WI, U.S.A, 04/2020
Using FRET-based Measurements of Protein Complexes to Determine Stoichiometry with the Job PlotFrancesca Mattiroli (1) , Yajie Gu (1,2) , Karolin Luger (1), (1) HHMI, University of Colorado Boulder, Dept. Chem. u. Biochem., Boulder, CO, USA , (2) Colorado State University, Dept. Biochem. u. Mol. Biol., Fort Collins, CO, USA, 09/2019
A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorusRead article
PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinaseRead article
Nat Chem Biol
Arrestin Recruitment to C-C Chemokine Receptor 5: Potent C-C Chemokine Ligand 5 Analogs Reveal Differences in Dependence on Receptor Phosphorylation and Isoform-Specific Recruitment BiasRead article
Binding pathway determines norepinephrine selectivity for the human β1AR over β2ARRead article
Amyloid formation of fish β-parvalbumin involves primary nucleation triggered by disulfide-bridged protein dimersRead article
Proc Natl Acad Sci U S A