Protein interactions
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Resources
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
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Studying the molecular mechanism of viral replication in real time using the CLARIOstar Plus with ACU
Marko Noerenberg (1) , Vincenzo Ruscica (2) , Alfredo Castello (1,2*), (1) MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland (UK) , (2) Department of Biochemistry, University of Oxford, South Parks road, OX1 3QU, Oxford, (UK) *Correspondence to: alfredo.castello@glasgow.ac.uk, 11/2020 - 348
Binding kinetics: high throughput assay for kinase inhibitors
Krumm A (1) , Georgi V (2) , Schiele F (2) , Fernández-Montalván A (2), (1) BMG LABTECH GmbH , (2) Bayer AG, Drug Discovery, Pharmaceuticals, Berlin, Germany, 06/2020 - 347
Ubiquitination monitoring in real-time: the fluorescence polarization-based method UbiReal
Tyler G. Franklin , Jonathan N. Pruneda, Oregon Health & Science University, Dept. of Molecular Microbiology and Immunology, Portland, OR, USA, 05/2020 - 346
Elucidating PROTAC MoA with live cell kinetic monitoring of ternary complex formation and target protein ubiquitination
Kristin Riching , Amy Landreman , Danette Daniels, Promega, Madison, WI, U.S.A, 04/2020 - 340
Using FRET-based Measurements of Protein Complexes to Determine Stoichiometry with the Job Plot
Francesca Mattiroli (1) , Yajie Gu (1,2) , Karolin Luger (1), (1) HHMI, University of Colorado Boulder, Dept. Chem. u. Biochem., Boulder, CO, USA , (2) Colorado State University, Dept. Biochem. u. Mol. Biol., Fort Collins, CO, USA, 09/2019
A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorus
Read articleNat Commun
PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase
Read articleNat Chem Biol
Arrestin Recruitment to C-C Chemokine Receptor 5: Potent C-C Chemokine Ligand 5 Analogs Reveal Differences in Dependence on Receptor Phosphorylation and Isoform-Specific Recruitment Bias
Read articleMol Pharmacol
Binding pathway determines norepinephrine selectivity for the human β1AR over β2AR
Read articleCell Res
Amyloid formation of fish β-parvalbumin involves primary nucleation triggered by disulfide-bridged protein dimers
Read articleProc Natl Acad Sci U S A
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