Measuring cell viability and death mechanisms is indispensable for testing new compounds, treatment regiments and other toxicological questions. Since cell-culture experiments are cost- and time-consuming the combination of assays is desirable to maximize data output of an experiment. Here, we present the multiplexing of cell viability with either apoptosis assays or with a reporter system indicating living cells, to obtain cell number-corrected viability values.
Using the fluorescent CellTiter-Blue™ assay which measures the cellular redox potential with the luminescent Caspase-Glo® 3/7 assay indicated the loss of viability and induction of apoptosis in Jurkat cellsupon addition of the Fas-Ligand, an apoptosis-inducer. Similar results were obtained using CellTier-Blue® with Apo-ONE, a substrate cleaved by caspases to a fluorescent molecule.
Relating viability measured by CellTiter-Glo® to a live cell reporter luciferase assay resulted in a stable viability signal in undisturbed cells irrespective of cell proliferation.
BMG LABTECH multi-mode plate readers offer the required sensitivity and flexibility to read multiple assays in one well.