Fluorescent quantification of low amounts of dsDNA using AccuBlue NextGen from Biotium

Alexis Madrid (1), Carl Peters (2) (1) Biotium Inc., Fremont, CA, (2) BMG LABTECH Inc., NC, USA 05/2017

Sensitive quantification of DNA in a sample is of vital importance to applications such as forensic DNA analysis where low amounts of DNA may be present. Similarly, accurate DNA quantification is paramount for Next Generation Sequencing (NGS), where precise amounts of DNA are required to enable this powerful tool in the investigation of genetic phenomena involved in mutations that lead to cancer or disease. Fluorescent quantification methods have proven accuracy and excellent sensitivity.


Here we show the performance of DNA quantification using AccuBlue™ NextGen reagent with detection by the CLARIOstar® microplate reader. It has the sensitivity to enable optimal performance of the AccuBlue NextGen dsDNA Quantification Kit. We obtained excellent linearity across all DNA concentrations tested and observed LOD values in 96 and 384 well plates that are better than previously reported. Thus this combination provides excellent sensitivity in DNA-quantification and the ability to conserve samples when needed.

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