The Dual-Luciferase® Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two-step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. The dual measurement of these two enzymes allows for an experimental measurement and a transfection control measurement to be done at the same time. This dual reporting of each sample allows a quantitative result based on the normalization of the Renilla luciferase (transfection control).
Using the DLR certification kit from Promega, BMG LABTECH microplate readers were tested regarding their performance in reading the DLR assay.
The Omega series of microplate readers as well as the CLARIOstar® and the PHERAstar® FS from BMG LABTECH has been granted DLReady™ certification based on the results published in this application note.