Dual emission detection for FP, FRET, TR-FRET, BRET, and dual glow assays
This greatly benefits assays like Fluorescence Polarization (FP), Forster resonance energy transfer (FRET), Time-Resolved-FRET (TR-FRET), Bioluminescence resonance energy transfer (BRET), and Dual Glow assays.
The benefits include faster measurement times (twice as fast) because it only has to read the plate once. But more importantly it eliminates read-to-read variations that can occur, such as different well volumes, concentrations, meniscus effects, or fluctuations in excitation energy. The result is an assay that is faster, is more sensitive, has a higher Z' factor, and has a lower %CVs.