Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Ubiquitination monitoring in real-time: the ﬂuorescence polarization-based method UbiRealTyler G. Franklin , Jonathan N. Pruneda, Oregon Health & Science University, Dept. of Molecular Microbiology and Immunology, Portland, OR, USA, 05/2020
Elucidating PROTAC MoA with live cell kinetic monitoring of ternary complex formation and target protein ubiquitinationKristin Riching , Amy Landreman , Danette Daniels, Promega, Madison, WI, U.S.A, 04/2020
Oxygen Dissociation Assay (ODA): spectrophotometric based screening platform for hemoglobin-O2 afﬁnity modiﬁersMira P Patel (1) , Vincent Siu (1) , Abel Silva-Garcia (1) , Qing Xu (2) , Zhe Li (2) , Donna Oksenberg (1), (1) Biology Department , (2) Chemistry Department, Global Blood Therapeutics Inc., South San Francisco, CA, 06/2019
A pH determination method suitable for high-throughput approaches based on spectral absorbanceAndrew Witschi (1) , Carl Peters (2), (1) Hudson Robotics Inc., Springfield, NJ , (2) BMG LABTECH, Cary, NC , 04/2019
Ellman’s assay for in-solution quantification of sulfhydryl groupsRucha Bhat , Gregory Grover, PhD, Contraline Inc., Charlottesville, VA, 11/2018
Calcium dynamically alters erythrocyte mechanical response to shearRead article
Biochim Biophys Acta Mol Cell Res
The protein-surfactant stoichiometry governs the conformational switching and amyloid nucleation kinetics of tau K18Read article
Eur. Biophys. J.
High-throughput assay for determining enantiomeric excess of chiral diols, amino alcohols, and amines and for direct asymmetric reaction screeningRead article
Physicochemical Characteristics of Protein Isolated from Thraustochytrid OilcakeRead article
Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformationRead article