Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Assessing parallelism using Parallel Line Analysis (PLA) in MARSMario Schneider (1), Michael Haitz (1), Klaus-Jürgen Ziegler (1), Carl Peters (2), (1) BMG LABTECH GmbH, Ortenberg, Germany; (2) BMG LABTECH Inc., Cary NC, USA, 02/2019
Development of a rapid HTRF insulin assayZ.J. Farino (1) , T.J. Morgenstern (1) , J. Vallaghe (2) , N. Gregor (2) , P. Donthamsetti (1) , P.E. Harris (1) , N. Pierre (2) , R. Freyberg (3) , F. Charrier-Savournin (2) , J.A. Javitch (1) , Z. Freyberg (1), (1) Columbia University , (2) Cisbio , (3) Yeshiva University, 04/2016
Fluorescence polarization based assay suitable for screening H-Prostaglandin D Synthase inhibitorsCarl Peters, BMG LABTECH, 07/2015
Using SPARCL technology to develop immunoassays for biomarker detection and pharmacokinetic studiesWenhua Xie (1) , Mark Cameron (1) , Carl Peters (2), (1) Lumigen , (2) BMG LABTECH, 06/2015
Cisbio's HTRF cortisol assay performed on the PHERAstar FSJean-Luc Tardieu , Francois Degorce, Cisbio, 03/2007
The prediction of thriving in elite sport: A prospective examination of the role of psychological need satisfaction, challenge appraisal, and salivary biomarkersRead article
J Sci Med Sport
Oxidative switch drives mitophagy defects in dopaminergic parkin mutant patient neuronsRead article
HIV-1 Tat promotes age-related cognitive, anxiety-like, and antinociceptive impairments in female mice that are moderated by aging and endocrine statusRead article
NLRP3 inflammasome inhibition with MCC950 improves insulin sensitivity and inflammation in a mouse model of frontotemporal dementiaRead article
The Role of Testosterone and Gibberellic Acid in the Melanization of Cryptococcus neoformansRead article