Enzyme activity
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Resources
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
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CLARIOstar determines activity of a moss-produced human acid alpha-glucosidase (GAA) in a fluorescence-based assay
Birgit Berg (1) , Anita Fischer (1) , Fabian Hässler (1) , Paulina Dabrowska-Schlepp (1) , Franka Maurer (2) , Stefanie Tintelnot (2), (1) Greenovation Biotech GmbH, Germany , (2) BMG LABTECH GmbH, Ortenberg, Germany, 08/2019 - 330
CLARIOstar Plus simplifies enzymatic reaction monitoring with its novel Enhanced Dynamic Range (EDR) technology
Mario Schneider, Jendrik Marbach , , BMG LABTECH GmbH, Ortenberg, Germany, 02/2019 - 328
A protein-based biosensor for mRNA
Alexander Cook , Christopher P. Toseland, School of Biosciences, University of Kent, UK, 12/2018 - 326
Fluorescence polarization-based RNA synthesis assay
Stefan Reich , Stephen Cusack, European Molecular Biology Laboratory, 10/2018 - 315
Assay development for essential enzyme activity in the tegument of live Schistosomes
Madhu Sundaraneedi (1) , Luke Becker (2) , Giovanni Abbenante (3) , Alex Loukas (2) , Grant Collins (1) , Mark Pearson (2), (1) School of Physical, Environmental and Mathematical Sciences, University of New South Wales, Australia , (2) Australian Institute for Tropical Health and Medicine, James Cook University, Australia , (3) BMG LABTECH Australia, 12/2017
Electrochemical screening of genoprotective and antioxidative effectiveness of Origanum vulgare L. and its functionality in the prevention of neurodegenerative disorders
Read articleTalanta
A double role of the Gal80 N terminus in activation of transcription by Gal4p
Read articleLife Sci Alliance
A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorus
Read articleNat Commun
Flipping the substrate preference of Hazara virus ovarian tumour domain protease through structure-based mutagenesis
Read articleActa Crystallogr D Struct Biol
Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus
Read articleSci Rep