Developing metabolic cell-based assays on the CLARIOstar
- Company: Agilent Technologies
- Interviewee: Ryan McGarrigle
- Products used: CLARIOstar
- Method: oxygen consumption and pH detection, real-time live-cell kinetic analysis
Ryan is part of the Cell Analysis Division at Agilent Technologies (former Luxcel Biosciences). Agilent develops fluorescence-based microplate assays to measure metabolism in different cell types. Assays include: oxygen consumption, extracellular acidification, and intracellular oxygen assay for measuring O2 concentration at a cellular level.
The CLARIOstar microplate reader is used in the lab for most of the assay development duties and offers a one-click solution for the Agilent metabolic assays. All Agilent measurement protocols come pre-installed on the reader and the MARS templates make data analysis very simple.