Learn about the numerous assays for cell death, toxicity, viability, and metabolic activity ...and how they can all be measured with one microplate reader.
Cell-based studies provide information about basic cellular processes. These include cell division, cell energy balance, control mechanisms, cell death regulation, differentiation or pathology. Cell-based methods are not only used for research: toxicity tests replace animal experiments and identify toxic substances in vitro. In addition, cell cultures have become important production organisms for medicines. For example, they produce insulin or monoclonal antibodies used as cancer therapeutics.
Modern cell biology analyses not only measure at a single time point but monitor biological changes over days in real time. To this end, the measurement chamber of a microplate reader needs controlled temperature and gas conditions. BMG LABTECH´s Atmospheric Control Unit regulates CO2 to stabilize the pH in carbonate buffered cell cultures. Additionally, O2 can be set to physiological conditions, which for mammalian cultures is typically below ambient concentrations. BMG LABTECH microplate readers further correct for non-homogenous distribution of a cell layer as they measure several points across the well instead of only reading in the center. To avoid biased results due to a undetected mycoplasma contamination, BMG LABTECH readers also offer the possibility of detecting existing contamination.
Setting up a cell biology experiment takes time. Therefore, novel assay solutions measure several parameters at once. The combination of different fluorescent, luminescent and chromogenic assays in one well results in multiple readouts. With a flexible microplate reader, all assays can be measured with only one instrument.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Viability assays: a comparison of luminescence-, ﬂuorescence-, and absorbance-based assays to determine viable cell countsAnn-Cathrin Volz , , BMG LABTECH, 77799 Ortenberg, Germany , 02/2022
NOX2 Activation Measured by ROS Production Using L-012 Chemiluminescent ProbeBhupesh Singla , Pushpankur Ghoshal , Huiping Lin , Qingqing Wei , Zheng Dong , Gábor Csányi , , Augusta University, Augusta, GA, U.S.A., 12/2021
Normalisation of Seahorse XFe96 metabolic assays to cell number with Hoechst stain using well-scan mode on the CLARIOstar PlusIan Hall, Cancer Research UK – Cambridge Institute, 09/2020
Autobioluminescent cells report real-time changes in cellular metabolism in response to pharmacological challengesKathryn Halter , Dan Close, 490 BioTech; Knoxville, TN, 02/2020
Cell-based assay detects residual nonsteroidal anti-inflammatory drugs (NSAIDs) in effluent of municipal wastewater treatmentK. Bernhard , C. Stahl , R. Martens , M. Frey, SIZ Zellkulturtechnik, c/o Hochschule Mannheim, Mannheim, Germany, 06/2018
The Importance of studying cells cultured in vitro under physiological conditions
Reactive oxygen species detection
Cell viability assays – Measure how happy your cells are
Cytotoxicity – These assays tell you what your cells don’t like
Bioactive peptides from broccoli stems strongly enhance regenerative keratinocytes by stimulating controlled proliferationRead article
A kinase-dead Csf1r mutation associated with adult-onset leukoencephalopathy has a dominant inhibitory impact on CSF1R signallingRead article
Development (Cambridge, England)
Chitosan Schiff bases-based polyelectrolyte complexes with graphene quantum dots and their prospective biomedical applicationsRead article
International journal of biological macromolecules
DLG2 impairs dsDNA break repair and maintains genome integrity in neuroblastomaRead article
Characterization of the Bifunctional Enzyme BioDA Involved in Biotin Synthesis and Pathogenicity in Aspergillus flavus