Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Expression of a stable green fluorescent protein mutant in group B streptococcus. Growth, detection and monitoring, with the CLARIOstarMatthew J. Sullivan , Glen C. Ulett, School of Medical Science and Menzies Health Institute Queensland, Grifﬁth University, Australia, 12/2018
Detection of plant-synthesized nanoparticles and their antibacterial capacitySalem W. and Schild S., University of Graz , Institute of Molecular Biosciences , BioTechMed-Graz , Austria, 03/2017
Lonza's kinetic kit for endotoxin detection using BMG LABTECH's microplate reader and MARS data analysisChris Quinlan , Carl Peters, BMG LABTECH, 07/2012
Growth of Campylobacter using a microplate reader equipped with ACUR.D. Haigh , J.M. Ketley, Department of Genetics , University of Leicester, 08/2011
Enzyme kinetic measurements for a combinatorial library of inhibitors of Pseudomonas elastaseGeorge Cathcart , Brendan Gilmore , Brian Walker, School of Pharmacy , QUB , Belfast, 10/2009
Population Analysis of Staphylococcus aureus Reveals a Cryptic, Highly Prevalent Superantigen SElW That Contributes to the Pathogenesis of BacteremiaRead article
A Bayesian non-parametric mixed-effects model of microbial growth curvesRead article
PLoS Comput Biol
Mammalian lipid droplets are innate immune hubs integrating cell metabolism and host defenseRead article
Can bacteriophage endolysins be nebulised for inhalation delivery against Streptococcus pneumoniae?Read article
Int J Pharm
Site specific mutations of GalR affect galactose metabolism in Streptococcus pneumoniaeRead article