7月 03, 2020
Ions can travel through the membrane using ion channels. Their activity is measured with fluorescent assays and a BMG LABTECH microplate reader.
Signaling
Cells not only receive extracellular cues, they also translate them into intracellular reactions. Extracellular signaling molecules comprise growth factors, hormones, ions, neurotransmitters, or cytokines.
Cellular reaction time covers from a few seconds to several hours. Accordingly, responses must either be triggered and measured instantly, or they need to be observed in living cells over a longer period of time. BMG LABTECH microplate readers provide injectors and a high sampling rate if fast reactions need to be captured. Further, the Atmospheric Control Unit regulates CO2 and O2 levels inside the reading chamber to provide a feel-good atmosphere for long-term cell experiments.
Signaling events rarely come alone, they take place simultaneously or in concert. In order to clearly characterize signaling events, BMG LABTECH devices offer the possibility of reading several assays in just one well.
Resources
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
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Time Resolved Fluorescence (TRF) immunoassay in 384-well format using a matched antibody pair kit and the PHERAstar FSX
Jamie Campbell (1) , Oliver Carney (2) , , (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Aylesbury, UK, 12/2020 - 349
Immuno-oncology biomarker measurement with high sensitivity and speed using SimpleStep ELISA kits and SPECTROstar Nano
Mallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 06/2020 - 342
Dual Channel Kinetic assays for detecting ligand bias at GPCRs
Paul Tewson (1) , Scott Martinka (1) , Kevin M. Harlen (1) , Thom Hughes (1) , Anne Marie Quinn (1) , Sam R.J. Hoare (2) , Carl Peters (3), (1) Montana Molecular, Bozeman, MT , (2) Pharmechanics, Owego, NY , (3) BMG LABTECH Inc., NC, USA, 11/2019 - 341
Highly sensitive ELISAs in 90-minutes: SimpleStep ELISA kits and the SPECTROstar Nano
Mallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 10/2019 - 333
Monitoring intracellular calcium using fluorescent dyes in a mid-throughput assay
Thomas P. Keeley , Ron Jacob , Giovanni E. Mann , King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine & Sciences, King’s College London, London SE1 9NH, UK, King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine and Sciences, King’s College London, 03/2019
Structural basis of GABAB receptor-Gi protein coupling
Read articleNature
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology
Read articleEBioMedicine
Targeting alveolar-specific succinate dehydrogenase A attenuates pulmonary inflammation during acute lung injury
Read articleFASEB journal : official publication of the Federation of American Societies for Experimental Biology
INPP4B protects from metabolic syndrome and associated disorders
Read articleCommunications biology
Pyrazole Agonist of the Apelin Receptor Improves Symptoms of Metabolic Syndrome in Mice
Read articleJournal of medicinal chemistry
