ProteaseTag active NE immunoassay: a rapid test to quantify neutrophil elastase levels in patients

Oliver Carney (1), Kelly Moffitt (2), Charlene Robb (2) (1) BMG LABTECH, (2) ProAxsis 02/2016

The neutrophil elastase is a biomarker of respiratory diseases such as cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). A simple, rapid and accurate test for neutrophil elastase levels in patient samples would be a significant medical benefit. Established methods do not specifically verify the presence of the enzyme leading to a high value of false positives. In this application note we show how to use the ProteaseTag™ Active Neutrophil Elastase Immunoassay from the company ProAxsis which is based in Northern Ireland.

The assay uses tags to specifically bind the enzyme of interest. Chromogenic detection is performed with the CLARIOstar® microplate reader which analysis the results and return qualified data automatically.

Protease levels can be determined in sputum sol, bronchoalveolar lavage, wound exudate or serum free cell culture.

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