Molecular biology

Our microplate readers answer your questions on nucleic acids: concentration, interaction with other nucleic acids or proteins, single nucleotide polymorphisms and expression of genes.

Molecular biology deals with biomolecules, their structure, function, and interactions. Since the discovery and description of DNA in the 1950s, we came to understand how DNA gets first transcribed into RNA and then translated into proteins. We further understand that the genome is not the only basis for a cell’s behavior, differentiation, and pathology. Epigenetic regulation, transcription regulation and non-coding RNAs are just as important.


Today, microplate reader assays are well-established tools to answer the questions that are still open. Luminescent reporter assays study gene regulation, interaction assays examine binding of nucleic acids to proteins and enzyme activity assays report on activities of polymerases or helicases.

 

Genomics is a multidisciplinary and essential part of modern biology.  Next generation sequencing (NGS) and SNP-genotyping are popular tools. The field is also closely associated with transcription and translation, investigating proteins and expression levels, both to directly inform studies but also as part of analysis workflows such as NGS.


Microplate reader also take measurements in preparation for PCRs and sequencing. They quantify nucleic acids in high throughput (96-1536 wells) and determine nucleic acid purity via absorbance spectra.

 

BMG LABTECH microplate readers offer solutions from simple quantification devices able to quantify DNA, RNA and protein efficiently and in high throughput, up to multi-functional devices suitable for all needs: reporter assays, binding assays, enzyme assays, no matter which detection mode.

Resources

Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.

    • Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing

      Vafaei, S;Allabush, F;Tabaei, SR;Male, L;Dafforn, TR;Tucker, JHR;Mendes, PM;[2021]

      ACS nano

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    • YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect

      Hou, Y;Zhang, Q;Pang, W;Hou, L;Liang, Y;Han, X;Luo, X;Wang, P;Zhang, X;Li, L;Meng, X;[2021]

      Cell death and differentiation

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    • Fine mapping identified the gibberellin 2-oxidase gene CpDw leading to a dwarf phenotype in squash (Cucurbita pepo L.)

      Ding, W;Wang, Y;Qi, C;Luo, Y;Wang, C;Xu, W;Qu, S;[2021]

      Plant Science

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    • A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage

      Zou, X;, ;Koh, G;Nanda, A;Degasperi, A;Urgo, K;Roumeliotis, T;Agu, C;Badja, C;Momen, S;Young, J;Amarante, T;Side, L;Brice, G;Perez-Alonso, V;Rueda, D;Gomez, C;Bushell, W;Harris, R;Choudhary, J;Jiricny, J;Skarnes, W;Nik-Zainal, S;[2021]

      Nature Cancer

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    • Regulation of IL-6/STAT-3/Wnt axis by nifuroxazide dampens colon ulcer in acetic acid-induced ulcerative colitis model: Novel mechanistic insight

      Ali, FEM;Elfiky, M;Fadda, WA;Ali, HS;Mahmoud, AR;Mohammedsaleh, ZM;Abd-Elhamid, TH;[2021]

      Life sciences

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