Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Normalisation of Seahorse XFe96 metabolic assays to cell number with Hoechst stain using well-scan mode on the CLARIOstar PlusIan Hall, Cancer Research UK – Cambridge Institute, 09/2020
Autobioluminescent cells report real-time changes in cellular metabolism in response to pharmacological challengesKathryn Halter , Dan Close, 490 BioTech; Knoxville, TN, 02/2020
FRET sensor assesses ATP levels in living plants experiencing low oxygen conditionsStephan Wagner (1,2) , Philippe Fuchs (1,2) , Thomas Nietzel (1,2) , Marlene Elsässer (1,2) , Markus Schwarzländer (1,2), (1) Institute of Plant Biology and Biotechnology, University of Münster, Germany , (2) Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Germany, 02/2018
The CLARIOstar with ACU exposes cells to ischemia-reperfusion conditions and monitors their oxygenationAndrea Krumm (1) , C. Carey (2) , M. Schwalfenberg (3) , J. Hynes (2) , R. Kettenhofen (3), (1) BMG LABTECH GmbH, Ortenberg , (2) Agilent Technologies, Cork, Ireland , (3) Ncardia, Cologne, Germany, 07/2017
Measuring changes in cellular metabolism by monitoring extracellular acidification and oxygen consumption in real-timeDavid Hoffman (1) , Carl Peters (2), (1) Cayman Chemical Ann Arbor, MI , (2) BMG LABTECH Inc. NC USA, 05/2017
Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson\'s disease: significance of SIRT3-mediated FOXO3 deacetylationRead article
Neural regeneration research
Single-cell analysis reveals immune modulation and metabolic switch in tumor-draining lymph nodesRead article
EpiMogrify Models H3K4me3 Data to Identify Signaling Molecules that Improve Cell Fate Control and MaintenanceRead article
Respiratory Supercomplexes Promote Mitochondrial Efficiency and Growth in Severely Hypoxic Pancreatic CancerRead article
Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1BRead article