Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Binding kinetics: high throughput assay for kinase inhibitorsKrumm A (1) , Georgi V (2) , Schiele F (2) , Fernández-Montalván A (2), (1) BMG LABTECH GmbH , (2) Bayer AG, Drug Discovery, Pharmaceuticals, Berlin, Germany, 06/2020
PHERAstar FSX certified for Transcreener assaysFranka Maurer (1) , Meera Kumar (2), (1) BMG LABTECH , (2) BellBrook Labs, 02/2016
Detection of tyrosine kinase activity in AlphaScreen modeFranka Ganske , Marjan Orban, BMG LABTECH, 12/2014
LanthaScreen TR-FRET tyrosine kinase and protein kinase C assayRandy Hoffman (1) , Megan Buros (1) , Kevin Kupcho (1) , EJ Dell (2), (1) Invitrogen , (2) BMG LABTECH, 12/2014
An AlphaScreen SureFire Phospho-ERK1/2 assayJD Brown , R. Mould , CJ Langmead, Heptares Therapeutics, 01/2011
Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor DesignRead article
The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin KRead article
A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA SignalingRead article
Int J Mol Sci
Targeting ALK2: An Open Science Approach to Developing Therapeutics for the Treatment of Diffuse Intrinsic Pontine GliomaRead article
J. Med. Chem.
Fluorescent Biosensor of CDK5 Kinase Activity in Glioblastoma Cell Extracts and Living CellsRead article