Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Dual Luciferase Reporter (DLR) assay certificationEJ Dell , Carl Peters, BMG LABTECH, 12/2014
Looking for a strong promoter for Physcomitrella patensLucas Schneider (1) , Manuel Hiss (1) , Stefanie Tintelnot (2) , Stefan Rensing (1), (1) University of Marburg , (2) BMG LABTECH, 11/2014
Simultaneous dual emission detection of luciferase reporter assaysMegan Dobbs (1) , Douglas Hughes (1) , Janaki Narahari (1) , Jae Choi (1) , Georgyi Los (1) , Brian Webb (1) , Eric Matthews (2) , Carl Peters (2), (1) Thermo Fisher Scientific , (2) BMG LABTECH, 03/2013
Multiplex analysis of inflammatory cytokines from primary human macrophages using a FLUOstar OmegaBrenda E. Mutch , Kate Willetts , Emma Timms , Brian MJ Foxwell, Kennedy Institute of Rheumatology, 12/2008
Rapid ultra-sensitive isothermal DNA detection using RPA technology and a BMG LABTECH microplate readerOlaf Piepenburg , Niall A. Armes, TwistDX Ltd., 08/2008
Mitoxantrone, pixantrone and mitoxantrone (2-hydroxyethyl)piperazine are toll-like receptor 4 antagonists, inhibit NF-κB activation, and decrease TNF-alpha secretion in primary microgliaRead article
Eur J Pharm Sci
Unique targeting of androgen-dependent and -independent AR signaling in prostate cancer to overcome androgen resistanceRead article
Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesisRead article
Polythiophenes with Cationic Phosphonium Groups as Vectors for Imaging, siRNA Delivery, and Photodynamic TherapyRead article
Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repairRead article
Nucleic Acids Res.