Cell viability and cytotoxicity
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Normalisation of Seahorse XFe96 metabolic assays to cell number with Hoechst stain using well-scan mode on the CLARIOstar PlusIan Hall, Cancer Research UK – Cambridge Institute, 09/2020
Autobioluminescent cells report real-time changes in cellular metabolism in response to pharmacological challengesKathryn Halter , Dan Close, 490 BioTech; Knoxville, TN, 02/2020
Calcium retention capacity assay evaluates inhibition of mitochondrial permeability transition poreM. Awais , D. Latawiec , R. Sutton, Liverpool Pancreatitis Research Group, Institute of Translational Medicine, University of Liverpool, UK, 11/2017
Real-time assessment of apoptosis and necrosisKevin Kupcho (1) , Carl Peters (2) , Andrew Niles (1), (1) Promega Corporation, Madison, WI , (2) BMG LABTECH Inc., NC, USA, 09/2017
The new Atmospheric Control Unit (ACU) for the CLARIOstar provides versatility in long-term cell-based assaysCarl Peters (1) , Tracy Worzella (2), (1) BMG LABTECH , (2) Promega, 02/2015
Role of Mac-1 integrin in generation of extracellular vesicles with antibacterial capacity from neutrophilic granulocytesRead article
J Extracell Vesicles
PAC proton-activated chloride channel contributes to acid-induced cell death in primary rat cortical neuronsRead article
In vivo safety profile of a CSPG4-directed IgE antibody in an immunocompetent rat modelRead article
Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regenerationRead article
Neural Regen Res
Anti-tumor effects of mevalonate pathway inhibition in ovarian cancerRead article