Learn about the numerous assays for cell death, toxicity, viability, and metabolic activity ...and how they can all be measured with one microplate reader.
Cell-based studies provide information about basic cellular processes. These include cell division, cell energy balance, control mechanisms, cell death regulation, differentiation or pathology. Cell-based methods are not only used for research: toxicity tests replace animal experiments and identify toxic substances in vitro. In addition, cell cultures have become important production organisms for medicines. For example, they produce insulin or monoclonal antibodies used as cancer therapeutics.
Modern cell biology analyses not only measure at a single time point but monitor biological changes over days in real time. To this end, the measurement chamber of a microplate reader needs controlled temperature and gas conditions. BMG LABTECH´s Atmospheric Control Unit regulates CO2 to stabilize the pH in carbonate buffered cell cultures. Additionally, O2 can be set to physiological conditions, which for mammalian cultures is typically below ambient concentrations. BMG LABTECH microplate readers further correct for non-homogenous distribution of a cell layer as they measure several points across the well instead of only reading in the center. To avoid biased results due to a undetected mycoplasma contamination, BMG LABTECH readers also offer the possibility of detecting existing contamination.
Setting up a cell biology experiment takes time. Therefore, novel assay solutions measure several parameters at once. The combination of different fluorescent, luminescent and chromogenic assays in one well results in multiple readouts. With a flexible microplate reader, all assays can be measured with only one instrument.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Normalisation of Seahorse XFe96 metabolic assays to cell number with Hoechst stain using well-scan mode on the CLARIOstar PlusIan Hall, Cancer Research UK – Cambridge Institute, 09/2020
Autobioluminescent cells report real-time changes in cellular metabolism in response to pharmacological challengesKathryn Halter , Dan Close, 490 BioTech; Knoxville, TN, 02/2020
Cell-based assay detects residual nonsteroidal anti-inflammatory drugs (NSAIDs) in effluent of municipal wastewater treatmentK. Bernhard , C. Stahl , R. Martens , M. Frey, SIZ Zellkulturtechnik, c/o Hochschule Mannheim, Mannheim, Germany, 06/2018
FRET sensor assesses ATP levels in living plants experiencing low oxygen conditionsStephan Wagner (1,2) , Philippe Fuchs (1,2) , Thomas Nietzel (1,2) , Marlene Elsässer (1,2) , Markus Schwarzländer (1,2), (1) Institute of Plant Biology and Biotechnology, University of Münster, Germany , (2) Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Germany, 02/2018
Calcium retention capacity assay evaluates inhibition of mitochondrial permeability transition poreM. Awais , D. Latawiec , R. Sutton, Liverpool Pancreatitis Research Group, Institute of Translational Medicine, University of Liverpool, UK, 11/2017
The Importance of studying cells cultured in vitro under physiological conditions
Reactive oxygen species detection
Cell viability assays – Measure how happy your cells are
Cytotoxicity – These assays tell you what your cells don’t like
Defective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbeRead article
Limiting protease production plays a key role in the pathogenesis of the divergent clinical isolates of Staphylococcus aureus LAC and UAMS-1Read article
Differential susceptibility of mycotoxin-producing fungi to distinct antifungal proteins (AFPs)Read article
Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytesRead article
A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansionRead article