Cell biology

Discover how cell-based microplate assays advance your research: from viability to cell death mechanisms, from glycolysis to respiration, from lytic assays to live cell assays, from one to multiple assays.

Cell-based studies provide information about basic cellular processes. These include cell division, cell energy balance, control mechanisms, cell death regulation, differentiation or pathology. Cell-based methods are not only used for research: toxicity tests replace animal experiments and identify toxic substances in vitro. In addition, cell cultures have become important production organisms for medicines. For example, they produce insulin or monoclonal antibodies used as cancer therapeutics.


Modern cell biology analyses not only measure at a single time point but monitor biological changes over days in real time. To this end, the measurement chamber of a microplate reader needs controlled temperature and gas conditions. BMG LABTECH´s Atmospheric Control Unit regulates CO2 to stabilize the pH in carbonate buffered cell cultures. Additionally, O2 can be set to physiological conditions, which for mammalian cultures is typically below ambient concentrations. BMG LABTECH microplate readers further correct for non-homogenous distribution of a cell layer as they measure several points across the well instead of only reading in the center. To avoid biased results due to a undetected mycoplasma contamination, BMG LABTECH readers also offer the possibility of detecting existing contamination.


Setting up a cell biology experiment takes time. Therefore, novel assay solutions measure several parameters at once. The combination of different fluorescent, luminescent and chromogenic assays in one well results in multiple readouts. With a flexible microplate reader, all assays can be measured with only one instrument.

Resources

Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.

    Ischemia-reperfusion proof-of-concept as example for Coronary Heart Diseases
    8月 09, 2019

    Mimicking the in vivo environment of coronary heart disease

    Coronary heart disease (CHD) is a complex condition that affects millions of people worldwide. There are many pathways and processes involved in the pathogenesis of this disease, and attempting to mimic these interactions in laboratory environments can prove troublesome. This blog article explains how ischemia and reperfusion models can be used to examine the complications of CHD and discover potential drug targets.

    Read more
    • Defective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbe

      Krachler, AM;Sirisaengtaksin, N;Monteith, P;Paine, CET;Coates, CJ;Lim, J;[2021]

      Virulence

      Read article
    • Limiting protease production plays a key role in the pathogenesis of the divergent clinical isolates of Staphylococcus aureus LAC and UAMS-1

      Rom, JS;Beenken, KE;Ramirez, AM;Walker, CM;Echols, EJ;Smeltzer, MS;[2021]

      Virulence

      Read article
    • Differential susceptibility of mycotoxin-producing fungi to distinct antifungal proteins (AFPs)

      Martínez-Culebras, P;Gandía, M;Boronat, A;Marcos, J;Manzanares, P;[2021]

      Food Microbiology

      Read article
    • Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytes

      Jang, S;Chapa-Dubocq, X;Tyurina, Y;St Croix, C;Kapralov, A;Tyurin, V;Bayır, H;Kagan, V;Javadov, S;[2021]

      Redox Biology

      Read article
    • A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion

      Gonçalves, CA;Larsen, M;Jung, S;Stratmann, J;Nakamura, A;Leuschner, M;Hersemann, L;Keshara, R;Perlman, S;Lundvall, L;Thuesen, LL;Hare, KJ;Amit, I;Jørgensen, A;Kim, YH;Del Sol, A;Grapin-Botton, A;[2021]

      Nature communications

      Read article