Ions can travel through the membrane using ion channels. Their activity is measured with fluorescent assays and a BMG LABTECH microplate reader.
Cell Signalling and its Therapeutic Implications
Cells not only receive extracellular cues, they also translate them into intracellular reactions. Extracellular signaling molecules comprise growth factors, hormones, ions, neurotransmitters, or cytokines.
Cellular reaction time covers from a few seconds to several hours. Accordingly, responses must either be triggered and measured instantly, or they need to be observed in living cells over a longer period of time. BMG LABTECH microplate readers provide injectors and a high sampling rate if fast reactions need to be captured. Further, the Atmospheric Control Unit regulates CO2 and O2 levels inside the reading chamber to provide a feel-good atmosphere for long-term cell experiments.
Signaling events rarely come alone, they take place simultaneously or in concert. In order to clearly characterize signaling events, BMG LABTECH devices offer the possibility of reading several assays in just one well.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Time Resolved Fluorescence (TRF) immunoassay in 384-well format using a matched antibody pair kit and the PHERAstar FSXJamie Campbell (1) , Oliver Carney (2) , , (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Aylesbury, UK, 12/2020
Immuno-oncology biomarker measurement with high sensitivity and speed using SimpleStep ELISA kits and SPECTROstar NanoMallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 06/2020
Dual Channel Kinetic assays for detecting ligand bias at GPCRsPaul Tewson (1) , Scott Martinka (1) , Kevin M. Harlen (1) , Thom Hughes (1) , Anne Marie Quinn (1) , Sam R.J. Hoare (2) , Carl Peters (3), (1) Montana Molecular, Bozeman, MT , (2) Pharmechanics, Owego, NY , (3) BMG LABTECH Inc., NC, USA, 11/2019
Highly sensitive ELISAs in 90-minutes: SimpleStep ELISA kits and the SPECTROstar NanoMallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 10/2019
Monitoring intracellular calcium using fluorescent dyes in a mid-throughput assayThomas P. Keeley , Ron Jacob , Giovanni E. Mann , King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine & Sciences, King’s College London, London SE1 9NH, UK, King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine and Sciences, King’s College London, 03/2019
Novel Interactions Involving the Mas Receptor Show Potential of the Renin-Angiotensin system in the Regulation of Microglia Activation: Altered Expression in Parkinsonism and DyskinesiaRead article
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
CPT1C negatively regulates the endocannabinoid hydrolase ABHD6 depending on nutritional statusRead article
British journal of pharmacology
Comparisons of neuroinflammation, microglial activation, and degeneration of the locus coeruleus-norepinephrine system in APP/PS1 and aging miceRead article
Journal of neuroinflammation
Identification of Key Receptor Residues Discriminating Human Chorionic Gonadotropin (hCG)- and Luteinizing Hormone (LH)-Specific SignalingRead article
International journal of molecular sciences
A Combination of BRD4 and HDAC3 Inhibitors Synergistically Suppresses Glioma Stem Cell Growth by Blocking GLI1/IL6/STAT3 Signaling AxisRead article
Molecular cancer therapeutics