October 11, 2019
Measure microbial growth using the „OD600“
Do you remember what you did in your microbiology practical during your studies? Clearly, I cannot recall every detail, but I still know that measuring the OD600 was an essential part of it: Draw a sample, place it into a cuvette, measure the OD at 600 nm and record the value along with the time. Ten years have passed since then and yet capturing the OD600 to determine microbial growth is more common than ever. Reason enough for us to shed light on why the method is used so intensively, how the method developed and what should be considered in the OD600 measurement.