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Whether studying a particular disease or a particular tissue the relevance of the data obtained from cell-based assays are always going to be affected by how closely the conditions used in the study recapitulate the physiological environment in vivo.

Fluorescence emission is a time-dependent process yielding a high number of photons within nanoseconds  upon excitation and then decreasing with increasing time. 

The selection of the optimal or wrong microplate, also called microtiter plate,  can significantly affect the performance of your assay. In these few lines, I will give you general guidelines that should help you choosing the best microplate for your assay.

When analyzing biological data in the microplate format, usually three items come into play: the microplate, the assay kit and the microplate reader. However, when the assay does not work properly or does not give the expected results, the blame is quite often put on the microplate reader. Probably the fact that it is the last ring of the chain or the most expensive item makes it easier to use it as a scapegoat.

BMG LABTECH application note 206 describes the performance of the PHERAstar FS in detection of intracellular calcium and dopamine receptor activation.

One of the capabilities that all BMG LABTECH microplate readers have is the script function which allows multiple functions to be performed without the need for user intervention.