A leukocyte adhesion assay performed on a BMG LABTECH microplate reader

DA Sanders, DS Charnock-Jones University of Cambridge 03/2009

A key component of the inflammatory response is the recruitment and extravasation of leukocytes into perivascular tissue. This process requires leukocyte interaction with vascular endothelium and consists of a multistep process including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration.


The adhesion of leukocytes to endothelial cells can be measured by addition of fluorophore labeled leukocytes to a monolayer of the endothelial cell line HUVECs, removal of non-adhered leukocytes and subsequent fluorescence detection of adhering cells.


This study shows how the assay can be measured on a BMG LABTECH microplate reader using the possibility to measure from the bottom of the well and to scan the well surface.


The results show good reproducibility and prove a rapid screening of the adhesion process. This easy to use assay can also be performed with primary neutrophils or PBMC if a more "in vivo-like" assay is required.

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