TR-FRET - Time-Resolved Fluorescence Resonance Energy Transfer

A special form of time-resolved fluorescence (TRF) is called time-resolved fluorescence resonance energy transfer (TR-FRET). TR-FRET is a FRET technique that uses lanthanide donors that have long emission half lives. A comparison meausrement of the two emitted wavelengths over time is calculated for a TR-FRET response. Examples of commercially available assays include such as HTRF®, LanthaScreen®, LANCE® and Transcreener® ADP2 TR-FRET Red Assay). TR-FRET assays are very robust (limited sensitivity to several types of assay interference) and are easily miniaturized.

BMG LABTECH's microplate readers have a unique Decay Curve Monitoring feature, a special assay development tool for time-resolved fluorescence, TR-FRET and AlphaScreen® assays. This technology is an indispensable tool for troubleshooting an assay or for fine-tuning parameters for optimal performance.

BMG LABTECH has published numerous application notes (AN) on using TR-FRET detection mode with various BMG LABTECH microplate readers including:

• Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH (AN 193)
• HTRF®IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus (AN 177)

The following BMG LABTECH microplate readers can be configured to perform TR-FRET measurements: