Fluorescence Intensity (FI)

Fluorescence intensity detection in microplate readers has been widely applied over the last two decades due to the vast development of new fluorophores. Typically, an optical system with a high power xenon flash lamp illuminates and excites the sample at a specific wavelength selected by a high performance optical filter.  As a result, the sample emits light and a second optical system collects the emitted light. Usually, the emitted light is of lower energy and thus is composed of a longer wavelength than the excitation light. The emitted light is collected and measured by the signal light detector, a photomultiplier tube (PMT).

BMG LABTECH has published numerous application notes (AN) on using fluorescence intensity detection mode with various BMG LABTECH microplate readers including:

• Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader (AN 187)
• The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers (AN 201)
• ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader (AN 197)
• Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation (AN 196)
• Analysis of Prostate Tumour Cell Invasion using BD FluoroBlok and FLUOstar OPTIMA (AN 144)

Click here for a complete list of fluorescence intensity application notes.

For more information on how to perform a simple fluorescence measurement with the Omega Series of microplate readers, take a look at the following video:

The following BMG LABTECH microplate readers can be configured to perform fluorescence measurements: