Fluorescence intensity detection in microplate readers has been widely applied over the last two decades due to the vast development of new fluorophores. Typically, an optical system with a high power xenon flash lamp illuminates and excites the sample at a specific wavelength selected by a high performance optical filter. As a result, the sample emits light and a second optical system collects the emitted light. Usually, the emitted light is of lower energy and thus is composed of a longer wavelength than the excitation light. The emitted light is collected and measured by the signal light detector, a photomultiplier tube (PMT).
BMG LABTECH has published numerous application notes (AN) on using fluorescence intensity detection mode with various BMG LABTECH microplate readers including:
Click here for a complete list of fluorescence intensity application notes.
For more information on how to perform a simple fluorescence measurement with the Omega Series of microplate readers, take a look at the following video:
The following BMG LABTECH microplate readers can be configured to perform fluorescence measurements: