Decay Curve Monitoring

Decay Curve Monitoring is a unique feature on the PHERAstar series of HTS microplate readers. It is a crucial assay development tool for time-resolved fluorescence, TR-FRET and AlphaScreen® assays. Time-resolved fluorescence PMTs and a direct photon measurement system allow for µsecond detection.

The Decay Curve Monitoring (DCM) feature was first employed in BMG LABTECH’s RUBYstar, the first dedicated HTRF® HTS microplate reader. It is now a standard feature on all PHERAstar HTS microplate readers and it can be used for TRF, TR-FRET and AlphaScreen® assays. Decay Curve Mointoring is a unique BMG LABTECH technology that is an indispensable tool for troubleshooting an assay or for fine-tuning parameters for optimal performance.

Using Decay Curve Moritoring, decay curves can be collected for one or two wavelengths with a user-selectable resolution down to 2 µs resolution. Assay performance can be improved by altering the measurement delay time or the signal integration time. Areas in the measurement protocol can also be identified and altered to increase the count rate. While other TR-FRET microplate readers simply give a value for the area under the curve, the PHERAstar series gives much more.