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MARS Data Analysis Software - Data Processing


Calculations Window Corrections Replicate Statistics TR-FRET Calculations Kinetic Calculations Standard Curve Calcuations Data Calculations Validations Spectrum Calculations Kinetic Spectrum Calculations Curve Smoothing Kinetic Curve Fitting Concentration Calcualtions Assay Quality User Defined Calcualtions Enzyme Kinetic Curve Scaling

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

MARS Data Analysis software offers comprehensive Data Processing possibilites according to the needs of the individual user.

When you click on the calcuation buttons to the left, you can see some examples of how you can use this powerful and intiuitve software.

MARS screen

Automatic Blank and Baseline Corrections
Corrections

MARS automatically blank corrects endpoint data. If kinetic data is collected, a baseline can be defined and corrected through MARS. Users then have the option of using the raw or corrected data to perform subsequent calculations.

Replicate Statistics
Replicate Statistics

MARS will intuitively allow statistical functions for replicates. For example, averages are automatically given for both the raw and blank corrected data. Statistics for other functions can be calculated after the function is performed (i.e. linear regression fits). Other statistics such as standard deviations and percent CVs are also available.

TR-FRET Calculations
TR-FRET Calculations
Kinetic Calculations
Kinetic Calculations

Applications such as calcium flux and Dual-Luciferase® require the collection of data points over a period of time. If kinetic data is present, you can define meausrement time ranges and MARS allows calculations with those ranges. Ranges can also be defined on the Signal Curve tab or through the Range Button. Calculations include the determination of the slope, sum, average, time-to-threshold and maximum. Calculation for defined ranges such as the sum or slope are readily deducted. Ranges for kinetic data are easily defined on the signal curve.Kinetic Calculations Graphs

EC and IC% Values
Standard Calculations Window

The half maximal effect and inhibitiory concentrations, EC50 and IC50, are important factors that are automatically calculated by MARS. In addition, MARS can calculate any EC or IC percentage value.

Fit Results

Data Calculations
Data Calculation
Results of Data Calculation
Validation for Pass/Fail
Pass-Fail Validation

MARS has the ability to calculate a pass/fail point and to view your data accordingly. This is perfect for viewing potential hits in HTS assays where many plates and samples are analyzed.

Pass Fail Microplate View

Spectrum Calculations
SPectrum Calculations
Spectrum Calcualtions Results
Kinetic Spectrum
Kinetic Spectrum Calculations

3d kinetic Spectrum Difference

Kinetic SPectrum Calculations Ratio

Curve Smoothing
Curve Smoothing

Curve Smoothing 3

Curve Smoothing Max

Kinetic Curve Fitting
Kinetic fitting
Concentration Calculations
Concentration Calculaiton
Assay Quality - Z' Factor, S/N Ratio, etc.
Assay Quality- Z' Calculations

MARS has several calculations that determine assay quality, including Z' factor and signal-to-noise (S/N) ratio.

Z' factor calculation is a coefficient for evaluating the quality of an HTS assay. This coefficient reflects both the dynamic range and the variation associated with the measured signal.

Assay Quality S/N

S/N ratios indicate the degree of confidence that a signal is real compared to the background noise of the instrument.

Formula Generator for User-Specific Calculations
Formula Generator

Being partially based on MATLAB®, MARS includes some of most common formulas, equations and calculations needed for analyzing data obtained from a microplate reader. However, many researchers have specific calculations that are only relevant to their data. MARS includes its own formula generator to allow for almost any user-specific calculation.

Enzyme Kinetic
Enzyme Kinetic Calculations
Enzyme Kinetics results
Curve Scaling
Curve Scaling
Curve Scaling