MARS Data Analysis Software
Data Display

There are two ways to view data within MARS Data Analysis Software:

Microplate View
Table View

Microplate View shows all of the wells of the microplate in either the numerical form or as a graphical representation if applicable (i.e. kinetic curves, absorbance spectra or display data in colors). Table View creates separate categories for each column and sorts the data according to a chosen column. Both options allow users to view, print and export their data according to their preferences (e.g. to Excel™).

 

 

 

3D Well Mapping

In cell-based assays, cells may not be distributed evenly over the entire well. A key feature on BMG LABTECH readers is well scanning, which scans the whole well creating a matrix of that well. A new way to view that data is with the 3D Well Mapping feature in MARS. This feature can also be used to view kinetic absorbance data three dimensionally. The figure below (on the right) shows a 3D well map of a Bradford absorbance spectrum taken over 60 seconds - 475 nm peak decreases and 595 nm peak increases.

Enzyme Kinetics

Michaelis-Menten Equation, Lineweaver-Burk, sScatchard, et al. After measuring an enzyme catalyzed fluorescence, luminescence or absorbance reaction, MARS can instantly analyze the data to determine enzymatic constants such as Km and Vmax. This software feature, when used in conjunction with BMG LABTECH's high speed UV/Vis spectrometer in the Omega and PHERAstar FS, will revolutionize microplate based enzyme kinetics.

MARS generated Michaelis-Menten plot of the reaction velocity for an esterase catalyzed pNPA reaction. Reaction curves can be found to the right of the plot. Km and Vmax were determined using the different methods, shown in the table below.

Plot	E1
	Km [µmol]	Vmax [µmol/min]
Michaelis-Menten	0.056	44.8
Linewaever-Burk	0.073	49.2
Eadie-Hofstee	0.063	46.4
Scatchard	0.068	47.9
Hanes	0.056	44.4

 

 

 

After viewing data, you can go on to data processing...