PHERAstar Plus - Technical Specifications

Detection Modes

Fluorescence Intensity - including FRET
Fluorescence Polarization
High-End AlphaScreen^® / AlphaLISA^®
Luminescence - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance

Measurement Modes

Top reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements
Well Scanning

Microplate Formats

Up to 1536-well plates, user-definable

Light Source

High energy xenon flashlamp
Solid state Laser for AlphaScreen^®

Detectors

Four photomultiplier tubes, optimized for different detection modes

Optic Modules Capacity

Up to five application specific optic modules built in

Z-Adjustment

Automatic focal height adjustment (0.1 mm resolution)

Spectral Range

230 - 750 nm or 230 - 900 nm for ABS, FI, FP, TRF, TR-FRET
230 - 750 nm for LUM

Sensitivity

FI:
0.5 pM Fluorescein (10 amol/well) (384)
2.0 pM Fluorescein (16 amol/well) (1536)

FP
1.0 mP SD at 1 nM Fluorescein (384)
3.0 mP SD at 1 nM Fluorescein (1536)

TRF:
25 fM Europium (0.5 amol/well) (384)
100 fM Europium (0.8 amol/well) (1536)

HTRF^®:
Reader Control Kit (Eu) after 18h (384)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)

LUM:
10 amol/well ATP (384)

AlphaScreen^®:
< 100 amol* (384)

ABS:
Dynamic range: ±0.000 - 4.000 OD
± 0.005 OD for OD range 0.0 to 2.0 (384)
± 0.010 OD for OD range 2.0 to 3.0 (384)

Reading Speed

1 flash
19 s (384), 37 s (1536)

10 flashes
41 s (384), 1 min 53 s (1536)

50 flashes
1 min 35 s (384), 5 min 29 s (1536)

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Incubation

+5°C above ambient up to 45°C

Software

Software package including Reader Control and MARS Data Analysis Software

Dimensions

Width: 44 cm, depth: 48 cm, height: 42 cm
Weight: 36 kg

Accessories

• Stacker
Mid-Throughput Microplate Handling System
Magazines for up to 50 plates
Continuous loading feature
Barcode reader

• THERMOstar
Microplate Incubator and Shaker
Shakes and incubates four microplates simultaneously
Incubation up to 56°C in 1°C steps
Heating plates above and below the microplates

• Optic Modules
Optimized for dyes, fluophores and specific assays
For all applications from UV to NIR
Customized optic modules available upon request

• Upgrades
Upgrades to include options such as additional detection modes are available. Please contact your local representative for more information.

* Limit of detection < 100 amol of biotinylated and phosphorylated polypeptide (P-Tyr-100 assay kit, PerkinElmer, #6760620C),
measured in white 384 small volume microplates (17 µL/well)