NEPHELOstar - Technical Specifications

Detection Mode

Nephelometry

Measurement Modes

Endpoint and Kinetic measurements

Microplate Formats

Up to 384-well plates

Light Source

Self-monitoring laser diode
Wavelength 635±10 nm
Stability <0.2% deviation
Lifetime 20,000 hours
Output: 1 mW
Selectable beam width: 1.5 to 3.5 mm
Selectable Intensity 0-100%
Scattering angle: Detects up to 80° full cone angle

Sensitivity

Depends on particle size and liquid properties
Silica detection (particle size 0.5 to 10 µm) 800 nM

Dynamic Range: 5 decades
Maximum measurement value (1s= 500,000 Relative Nephelometry Units)

Reading Speed

Depend on assay conditions and liquid surface stability
23 s (96), 66 s (384)

Reagent Injection

Up to 3 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+5°C above ambient up to 38°C

Software

Software package including Reader Control and MARS Data Analysis software

Dimensions

Width: 44 cm, depth: 48 cm, height: 33 cm
Weight: 25 kg

Accessories

• Stacker
Mid-Throughput Microplate Handling System
Magazines for up to 50 plates
Continuous loading feature
Barcode reader

• THERMOstar
Microplate Incubator and Shaker
Shakes and incubates four microplates simultaneously
Incubation up to 56°C in 1°C steps
Heating plates above and below the microplates

• Upgrades
Upgrades to include options such as reagent injectors, gas vent etc. are available. Please contact your local representative for more information.