FLUOstar OPTIMA FL - Technical Specifications

Detection Modes

Fluorescence Intensity - including FRET
Time-Resolved Fluorescence - including DELFIA^®
Luminescence (flash and glow) - including BRET

Measurement Modes

Top reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Ratiometric measurements
Well Scanning

Microplate Formats

6 to 384-well plates, user-definable

Light Source

High energy xenon flashlamp

Detector

Side window photomultiplier tube

Optical Filters

Excitation and emission filter wheels for 8 filters each

Spectral Range

240 to 740 nm

Sensitivity

FI
< 1 fmol / well Fluorescein

TRF
< 70 amol/well Europium

LUM
< 30 amol/well ATP
DLReady certified

Read Times

Flying mode: 15 s (96), 30 (384)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

None or +5°C above ambient up to 45°C or 60°C

Software

Software package including Reader Control and MARS Data Analysis software

Dimensions

Width: 44 cm, depth: 48 cm, height: 26 cm
Weight: 26 kg

Accessories

• Stacker
Mid-Throughput Microplate Handling System
Magazines for up to 50 plates
Continuous loading feature
Barcode reader

• THERMOstar
Microplate Incubator and Shaker
Shakes and incubates four microplates simultaneously
Incubation up to 56°C in 1°C steps
Heating plates above and below the microplates

• Filters
Optimized for dyes, fluophores and specific assays
Filters for all applications from UV to NIR
Customized filters available upon request

• Upgrades
Upgrades to a higher model or to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information.