PHERAstar FS - Technical Specifications

Detection Modes

Fluorescence Intensity - including FRET
Fluorescence Polarization
High-End AlphaScreen^® / AlphaLISA^®
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra

Measurement Modes

Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements
Well Scanning

Microplate Formats

Up to 3456-well plates, user definable

Light Sources

High energy xenon flashlamp
Nitrogen Laser for TRF and TR-FRET
Solid state Laser for AlphaScreen^®

Detectors

Four photomultiplier tubes, optimized for different detection modes

Optic Modules Capacity

Up to six application specific Optic Modules built in

Z-Adjustment

Automatic focal height adjustment (0.1 mm resolution)

Spectral Range

230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
220 - 1000 nm for ABS (Absorbance Spectrometer)

Sensitvity

FI (top)
0.4 pmol/L sodium fluorescein (8 amol/well) (384)
2.0 pmol/L sodium fluorescein (16 amol/well) (1536)

FI (bottom)
5.0 pmol/L sodium fluorescein (0.2 fmol/well) (384)

FP
1.0 mP SD at 1 nM sodium fluorescein (384)
3.0 mP SD at 1 nM sodium fluorescein (1536)

TRF
25 fmol/L europium (0.5 amol/well) (384)
100 fmol/L europium (0.8 amol/well) (1536)

HTRF®
Reader Control Kit (Eu) after 18h (384)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)

LUM
10 amol / well ATP (384)

AlphaScreen^®
< 100 amol / well* (384)

ABS with Spectrometer
Spectral range: 220 - 1000 nm
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

1 flash
14 s (384), 27 s (1536)

10 flashes
38 s (384), 1 min 52 s (1536)

50 flashes
1 min 29 s (384), 5 min 18 s (1536)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Barcode Reader

Up to three integrated barcode readers

Incubation

+5°C above ambient up to 45°C

Software

Software package including Reader Control and MARS Data Analysis Software

Dimensions

Width: 45 cm, depth: 51 cm, height: 47 cm
Weight: 49 kg

Accessories

• Stacker
Mid-Throughput Microplate Handling System
Magazines for up to 50 plates
Continuous loading feature
Barcode reader

• THERMOstar
Microplate Incubator and Shaker
Shakes and incubates four microplates simultaneously
Incubation up to 56°C in 1°C steps
Heating plates above and below the microplates

• Optic Modules
Optimized for dyes, fluophores and specific assays
For all applications from UV to NIR
Customized optic modules available upon request

• Upgrades
Upgrades to include options such as additional detection modes, reagent injectors, etc. are available. Please contact your local representative for more information.

* Limit of detection < 100 amol of biotinylated and phosphorylated polypeptide (P-Tyr-100 assay kit, PerkinElmer, #6760620C),
measured in white 384 small volume microplates (17 µL/well)