POLARstar Omega - Technical Specifications

Detection Modes

Fluorescence Intensity - including FRET
Fluorescence Polarization
AlphaScreen^® / AlphaLISA^®
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra

Measurement Modes

Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Ratiometric measurements
Well Scanning

Microplate Formats

6 to 1536-well plates, user-definable

Light Source

High energy xenon flashlamp

Detectors

Two matched side window photomultiplier tubes

Optical Filters

Excitation and emission filter wheels for 8 filters each

Spectral Range

240 - 740 nm or 240 - 900 nm
Absorbance Spectrometer: 220 - 850 nm

Sensitivity

FI
< 0.2 fmol/well Fluorescein

FP
< 5 mP SD at 1 nM Fluorescein

TRF
< 30 amol/well Europium

High-End TRF for Omega
< 3 amol/well Europium

LUM
20 amol/well ATP
DLReady certified

AlphaScreen^®
< 100 amol* (384)

ABS with Spectrometer
Spectral range: 220 - 850 nm
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

Flying mode:
9 s (96), 16 s (384)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+5°C above ambient up to 45°C or 60°C

Software

License-free software package including Reader Control and MARS Data Analysis Software

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm
Weight: 28 kg

Software

Software package including Reader Control and MARS Data Analysis software

Accessories

• Stacker
Mid-Throughput Microplate Handling System
Magazines for up to 50 plates
Continuous loading feature
Barcode reader

• THERMOstar
Microplate Incubator and Shaker
Shakes and incubates four microplates simultaneously
Incubation up to 56°C in 1°C steps
Heating plates above and below the microplates

• Filters
Optimized for dyes, fluophores and specific assays
Filters for all applications from UV to NIR
Customized filters available upon request

• Upgrades
Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information.

* Limit of detection < 100 amol of biotinylated and phosphorylated polypeptide (P-Tyr-100 assay kit, PerkinElmer, #6760620C),
measured in white 384 small volume microplates (17 µL/well)