FLUOstar OPTIMA ABS - Filter-based absorbance microplate reader

Absorbance

The FLUOstar OPTIMA ABS absorbance microplate reader allows users the flexibility to upgrade to detect fluorescence and luminescence. If your lab can envision the need to detect luciferase or GFP, this platform can be quickly upgraded with those detection modes. The FLUOstar OPTIMA ABS absorbance microplate reader has other features most basic ELISA readers lack. It can be equipped with reagent injectors to initiate fast kinetic reactions, like enzyme assays. All OPTIMA microplate readers can accept, e.g. a gas-vent and incubation hardware, so cellular measurements can be performed at 5% CO2 and at 37°C. This is useful for cell proliferation and cytotoxicity assays, the MTT assay, apoptosis and cell migration as well as bacterial growth kinetics.

THe FLUOstar OPTIMA ABS absorbance microplate reader, as well as all BMG LABTECH microplate readers, come with user-friendly and intuitive reader control software as well as comprehensive data analysis software, MARS. It allows one-click calculation of DNA concentration, standard curve plots, error bars, standard deviations, IC50 / EC50s, live-dead ratios and Vmax and Km for enzyme kinetic calculations. It's easy enough that users will no longer have to print their data and enter it into another format or create their own standard curves in a dedicated data analysis software. After data is analyzed, it is only a simple one-step process to any data from the FLUOstar OPTIMA ABS absorbance microplate reader into Microsoft Excel® format. BMG LABTECH's advanced hardware, combined with our powerful data analysis tools, result in a detection synergy that provides better data, faster.

FLUOstar OPTIMA ABS Applications

The FLUOstar OPTIMA ABS absorbance microplate reader performs UV/Vis absorbance in any plate format from 6- to 96- or 384-wells.
Every absorbance microplate reader available should be able to measure Bradford, MTT and ELISA assays and the FLUOstar OPTIMA ABS does this well.
What sets this absorbance plate reader apart from other instruments is its abilityto detect wavelengths as low as 240 nm, low enough for NADH detection or DNA and protein 260/280 measurements. Nearly every other ELISA reader uses a lamp that stops producing light in the blue wavelength range, so they can't detect DNA at 260 nm. All FLUOstar OPTIMA microplate readers have xenon lamps that emit well into the lower UV. This low UV capability means it can be used for nearly all microplate based spectroscopy assays.

For BMG LABTECH application notes that can be performed on the FLUOstar OPTIMA ABS microplate reader, please click here.

Detection Modes	UV/Vis absorbance
Measurement Modes	Endpoint and Kinetic measurements Sequential Multi Excitation measurements Ratiometric measurements Well Scanning
Certified for	DLReady™
Microplate Formats	6 to 384-well plates, user-definable
Microplate Carrier	Robot compatible
Light Sources	High energy xenon flash lamp
Detectors	Low noise photomultiplier tube
Wavelength Selection	Optical Filters: Ex and Em wheels for 8 filters each
Optical Filters	Excitation and emission filter wheels for 8 filters each
Optical Path Guides	Top: Liquid filled light guides Bottom: Fiber optics
Spectral Range	240 to 740 nm or 240 to 900 nm for ABS
Sensitivity*	ABS Dynamic range: ±0.000 - 4.000 OD Reproducibility: ±0.010 OD for 0 - 2 OD range
Read Times	Flying mode 15 s (96), 30 s(384)
Reagent Injection	Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 350 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing
Shaking	Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent	System to inject an atmosphere or to pull a vacuum into the reader
Incubation	None or +5°C above ambient up to 45°C or 60°C
Software	Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant
Dimensions	Width: 44 cm, depth: 48 cm, height: 26 cm; weight: 26 kg
Accessories	Optional Accessories
Stacker	Magazines for up to 50 plates - continuous loading feature
THERMOstar	Microplate Incubator and Shaker
Optical Filters	Optimized for dyes, fluorophores and specific assays Filters for all applications from UV to NIR Customized filters available upon request
Upgrades	Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information.
*	LOD = 3 x SD (20 blanks) / slope (6 pt std curve) Microplatets: clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates

Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.

Additional Accessories:
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Additional Links: Applications Compatibility BMG LABTECH Software

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