PHERAstar FS
PHERAstar Plus
POLARstar Omega
FLUOstar Omega
LUMIstar Omega
SPECTROstar Omega
POLARstar OPTIMA
FLUOstar OPTIMA
FLUOstar OPTIMA ABS
FLUOstar OPTIMA F
FLUOstar OPTIMA FL
LUMIstar OPTIMA
NOVOstar
NEPHELOstar
RUBYstar
PHERAstar FS
PHERAstar Plus
POLARstar Omega
FLUOstar Omega
LUMIstar Omega
SPECTROstar Omega
POLARstar OPTIMA
FLUOstar OPTIMA
FLUOstar OPTIMA ABS
FLUOstar OPTIMA F
FLUOstar OPTIMA FL
LUMIstar OPTIMA
NOVOstar
NEPHELOstar
RUBYstar
Detection Modes
Fluorescence Intensity - including FRET
Fluorescence Polarization
High-End AlphaScreen®/AlphaLISA®
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra
Fluorescence Intensity - including FRET
Fluorescence Polarization
High-End AlphaScreen®/AlphaLISA®
Luminescence - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance
Measurement Modes
Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements
Well Scanning
Top reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements
Well Scanning
Certified for
HTRF®
LanthaScreen® Certified Plus
Transcreener® far red FP
Transcreener® red FI
Transcreener® red TR-FRET
HTRF®
LanthaScreen® Certified Plus
Transcreener® far red FP
Transcreener® red FI
Transcreener® red TR-FRET
Microplate Formats
Up to 3456-well plates, user-definable
Up to 1536-well plates, user-definable
Microplate Carrier
Single
Single
Light Sources
High energy xenon flash lamp
Nitrogen Laser for TRF and TR-FRET
Solid state Laser for AlphaScreen®
High energy xenon flash lamp
Solid state Laser for AlphaScreen®
Detectors
Four photomultiplier tubes, optimized for different detection modes
Four photomultiplier tubes, optimized for different detection modes
Optic Modules Capacity
Up to six application specific Optic Modules built in
Up to five application specific Optic Modules built in
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Automatic focal height adjustment (0.1 mm resolution)
Spectral Range
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
220 - 1000 nm for ABS
230 - 750 nm or 230 - 900 nm for ABS, FI, FP, TRF, TR-FRET
230 - 750 nm for LUM
Sensitivity
FI (top)
0.4 pmol/L sodium fluorescein (8 amol/well) (384)
2.0 pmol/L sodium fluorescein (16 amol/well) (1536)
FI (bottom)
5.0 pmol/L sodium fluorescein (0.2 fmol/well) (384)
FP
1.0 mP SD at 1 nM sodium fluorescein (384)
3.0 mP SD at 1 nM sodium fluorescein (1536)
TRF
25 fmol/L europium (0.5 amol/well) (384)
100 fmol/L europium (0.8 amol/well) (1536)
HTRF®
Reader Control Kit (Eu / Tb)
Delta F 1050 % (Eu) and 1150 % (Tb) (high calibrator)
Delta F 95 % (Eu) and 110 % (Tb) (low calibrator)
LUM
10 amol / well ATP (384)
AlphaScreen®
< 100 amol / well* (384)
ABS with Spectrometer
Spectral range: 220 - 1000 nm
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
FI
0.5 pM Fluorescein (10 amol/well) (384)
2.0 pM Fluorescein (16 amol/well) (1536)
FP
1.0 mP SD at 1 nM Fluorescein (384)
3.0 mP SD at 1 nM Fluorescein (1536)
TRF
25 fM Europium (0.5 amol/well) (384)
100 fM Europium (0.8 amol/well) (1536)
HTRF®
Reader Control Kit (Eu) after 18h (384)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)
LUM
10 amol/well ATP (384)
AlphaScreen®
< 100 amol* (384)
ABS
Dynamic range: ±0.000 - 4.000 OD
± 0.005 OD for OD range 0.0 to 2.0 (384)
± 0.010 OD for OD range 2.0 to 3.0 (384)
Read Times
1 flash
14 s (384), 27 s (1536)
10 flashes
38 s (384), 1 min 52 s (1536)
50 flashes
1 min 29 s (384), 5 min 18 s (1536)
1 flash
19 s (384), 37 s (1536)
10 flashes
41 s (384), 1 min 53 s (1536)
50 flashes
1 min 35 s (384), 5 min 29 s (1536)
Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Linear, orbital, and double-orbital with user-definable time and speed
Integrated Barcode Reader
Up to three integrated barcode readers
Incubation
+5°C above ambient up to 45°C
+5°C above ambient up to 45°C
Software
License-free software package including Reader Control and MARS Data Analysis Software
License-free software package including Reader Control and MARS Data Analysis Software
Dimensions
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
Width: 44 cm, depth: 48 cm, height: 42 cm; weight: 36 kg
Accessories
Optional Accessories
Optional Accessories
Stacker
Magazines for up to 50 plates - continuous loading feature
Magazines for up to 50 plates - continuous loading feature
THERMOstar
Microplate Incubator and Shaker
Microplate Incubator and Shaker
Optic Modules
Available for all applications
Available for all applications
Upgrades
Upgrades to include options such as additional detection modes, reagent injectors, etc. are available. Please contact your local representative for more information.
Upgrades to include options such as additional detection modes, etc. are available. Please contact your local representative for more information.
HTRF is a registered trademark of Cisbio International.
AlphaScreen is a registered trademark of PerkinElmer, Inc.
LanthaScreen is a registered trademark of Invitrogen Corp.
Transcreener is a patented technology of BellBrook Labs.