The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok™ tumour cell invasion system. In this application note we show that the results are comparable to the traditional invasion assays. The introduced method is fast, saving time and labour. The FLUOstar OPTIMA is able to take multiple readings over time at a user defined temperature. The FluoroBlok™ assay can also be performed on other readers from BMG LABTECH, including the PHERAstar FS, the FLUOstar and POLARstar Omega, and the NOVOstar.