FLUOstar Omega - Multi-mode microplate reader - including full UV/Vis absorbance spectrum
The Best Drug Discovery Product 2010, FLUOstar Omega, is a multi-mode microplate reader featuring BMG LABTECH's unique Tandem Technology. It utilizes high speed UV/Vis spectrometer based absorbance and ultra sensitive filter-based detection in all detection modes such as:
- UV/Vis Absorbance - measurement of complete spectra or individual wavelengths
- Fluorescence Intensity - including FRET
- Time-Resolved Fluorescence - including DELFIA®
- TR-FRET - including HTRF® and LanthaScreenTM
- AlphaScreen® / AlphaLISA®
- High-Performance Luminescence (flash and glow) - including BRET
Advanced Assay Technology Package for the FLUOstar Omega:
BMG LABTECH engineers have designed a specialized measurement mode and optical system to provide the Omega multimode microplate readers with AlphaScreen® / AlphaLISA® performance that rivals any HTS microplate reader. For the first time, experience fantastic AlphaScreen® / AlphaLISA® performance on a mid-range multi-mode microplate reader.
Furthermore, Advanced Performance TRF is part of the Omega's optional Advanced Assay Technology Package. It utilizes an optional high-end TRF optical reading head that incorporates an assay specific dichroic mirror. Combined with the high intensity xenon flash lamp, assay optimized filters and adjustable gain, the Omega high-end TRF reading head allows the Omega to outperform any microplate reader in the mid-range market.
Additional Features:
The FLUOstar Omega is based on the successful FLUOstar Optima multi-mode microplate reader and has adopted and enhanced mature hardware technology and outstanding software features:
- Top and bottom reading
- Onboard "smart" injectors dispense reagents and initiate kinetic events (e.g. monitoring protein denaturation or the formation of different products in an enzymatic assay over time)
- Temperature control up to 65°C
- Microplate capacity up to 1536-well format
- Impressive well-scanning feature allows cell-based assays to be performed with ease
- Automatic optical head recognition and optics switching
- Stacker and robot compatibility
- Intuitive test protocol set-up and comprehensive data analysis with MARS software
- Template manager for transferring standards, building complex data processing protocols and using default templates
- Versatile kinetic software features for endpoint, long-term and fast kinetic measurements
- Real-time kinetic monitoring
- "QuickStart" mode allows users to read plates with just 3 mouse clicks
- Unique gas control functions via the microprocessor controlled Atmospheric Control Unit (ACU), which allows for fully regulated individual control of O2 (1% to 19%) and CO2 (0% to 20%) within the microplate reader. Alternatively, BMG LABTECH offers an additional purge gas vent to replace the internal atmosphere of the microplate reader chamber with an inert gas (e.g. CO2, Ne or Ar).
- With the LVis Plate, the FLUOstar Omega micropalte reader is also capable of measuring low volume samples in a simple absorbance assay like DNA, RNA and protein concentration. The LVis Plate can measure 16 low volume samples such as DNA, RNA, or Protein. In addition, the built-in cuvette holder allows for larger sample measurements, while the optional quality control optical density (OD) and holmium oxide filters are fantastic for verifying instrument performance testing
- Upgradeable to POLARstar Omega with Fluorescence Polarization and Simultaneous Dual Emission detection
FLUOstar Omega applications include:
- Monitoring absorbance changes in cellular co-factors NADH and NADPH allowing the determination of enzymatic activity (AN170)
- ELISA, DNA and Bradford protein quantitation assays (AN169)
- Reaction optimization (metallation of porphyrin in twelve different solvents) by parallel kinetic studies (AN178)
- Aggregation assays with e.g. Thioflavin T (AN212, 2.995)
- Evaluation of PARP inhibitors (AN171)
- Measuring mitochondrial Membrane potential (AN175)
- Reporter gene assays: GFP, YFP, luciferase, dual luciferase, BRET (AN165)
- ORAC, Determination of antioxidant activity (AN197), oxygen consumption (AN207) and ROS determination (2.1002)
- Protein, DNA and RNA quantification (e.g. Bradford, Absorbance 260/280, Quant-iT PicoGreen Assay) (AN158, AN168, 2.994)
Additionally, a wide variety of applications can benefit from the Atmospheric Control Unit (ACU) for O2 / CO2 in conjunction with the FLUOstar Omega multi-mode microplate reader, including:
- Mammalian Cell Assays
- Stem Cell Assays
- Hypoxia
- Cell Viability (AN 159, AN 214)
- Cell Proliferation (AN 195)
- Cell Growth (AN 217, AN 155)
- Migration Studies (AN 204)
- Invasion Assays (AN 144)
- Intracellular pH (Assay Technology ph-XTRA)
- Mitochondrial Studies (AN 207)
- Angiogenesis
- Viral Uptake
- Bacterial Studies (AN 155, AN 207, AN 189, AN 199)
- Ischemia / Reperfusion Injury
The FLUOstar Omega is a modular multi-mode microplate reader platform for flexible assay development and cellular analysis that allows versatility in choice of assay read out and detection mode.
For more information and complete technical specifications, click here. (not optimized for mobile)
HTRF is a registered trademark of Cisbio international.
AlphaScreen, AlphaLISA, and DELFIA are registered trademarks of Perkin Elmer Inc.
LanthaScreen is a trademark of Invitrogen Corp.
Transcreener is a patented technology of BellBrook Labs.
Quant-iT and PicoGreen are trademarks of Invitrogen Corp.