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Quantifying double-stranded DNA with fluorescent dyes: Qubit™ on BMG LABTECH instruments

Andrea Krumm, Mark Gröne und Franka Maurer (BMG LABTECH GmbH, Ortenberg, Germany), 07/2017

The quantification of DNA is a standard laboratory activity and pivotal for subsequent applications such as next generation sequencing (NGS). The widely used Qubit™ nucleic acid staining dyes are analyzed on a Qubit™ fluorometer. It measures fluorescence intensity directly in sample preparation tubes and automatically calculates the DNA-concentration based on two standards measured in parallel. This provides an easy, quick and intuitive way to quantitate dsDNA. However, throughput is limited using the Qubit™ fluorometer as only one sample can be analyzed at a time.

This application not presents how the Qubit® dsDNA HS fluorophore can be used to quantitate dsDNA on a BMG LABTECH microplate reader. The Qubit kit read on the FLUOstar® Omega, CLARIOstar® and PHERAstar® resulted in a high linearity (R2 > 0.9999) and quantification of DNA dilutions of 50 pg/μl-100 ng/μl. Detecting Qubit chemistry on BMG LABTECH microplate readers increases throughput while keeping the simplicity of the quantification method.