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Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus

U-C. Hipler, U. Wollina, Department of Dermatology, Friedrich-Schiller-Universität, Jena, Germany; D. Denning, Department of Microbiology, University of Manchester, UK; B. Hipler, BMG LABTECH, Germany, 10/2002

The intracellular ROS formation is measured using 2',7'-dichlorfluorescein  diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2)