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Application Notes sorted by Assays

Molecular Biology
Measurement of DNA, ATP, Protein
[211 - 2.700]
Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus
Ferneyhough B, Parkinson N
BMG LABTECH 2011
Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format.
Article
[001 - 6.330]
DNA measurements in low volume samples, microplates and cuvettes on the SPECTROstar Nano
Groene M, Ganscke F, Wark C, Dell EJ
BMG LABTECH 2011
Three different ways to measure DNA samples in the SPECTROstar Nano are in detail explained and advantages of each option as well as sensitivity data are presented.
Article
[213 - 2.720]
The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH
Miriam Uppill, Antony Sheehan and Ron Osmond
BMG LABTECH 2011
Fluorescent based ELISA with only one wash step measured on the POLARstar Omega
Article
[202 - 4.340]
Promega's ADP-Glo(TM) Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega
Ganske, F, Dell, EJ, Shultz S
BMG LABTECH 2009
A homogeneous luminescent assay from Promega to detect ADP
Article
[201 - 2.660]
The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers
Franka Ganske, BMG LABTECH, Offenburg, Germany; EJ Dell, BMG LABTECH, NC, USA / Meera Kumar, BellBrook Labs, Madison, USA
BMG LABTECH 2009
The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay
Article
[196 - 2.620]
Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation
Muller RE, Siderovski DP, Kimple AJ, Dell EJ
BMG LABTECH 2009
Alternative, non-radiological method to assess heterotrimeric G protein activation
Article
[190 - 7.130]
Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus
Franka Ganske and Keith Ansell
BMG LABTECH 2009
Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented.
Article
[183 - 1.180]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP
Article
[182 - 1.170]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's PHERAstar Plus HTS Microplate Reader
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP
Article
[180 - 1.160]
High-throughput protein-DNA affinity measurements using fluorescence anisotropy
Veprintsev DB
BMG LABTECH 2008
The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied.
Article
[176 - 2.570]
Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA
Olaf Piepenburg and Niall A. Armes
BMG LABTECH 2008
The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format
Article
[170 - 6.210]
Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
Dell EJ
BMG LABTECH 2008
UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm.
Article
[169 - 6.200]
Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation
Dell EJ and Ganske F
BMG LABTECH 2008
Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented.
Article
[168 - 6.190]
Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
Ganske F
BMG LABTECH 2008
DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented.
Article
[166 - 2.540]
Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
Tracey Madgett, University of the West of England, Bristol
BMG LABTECH 2008
Protein determination using a fluorescent assay from Invitrogen
Article
[164 - 2.530]
Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation
Sarah Shammas and Ann-Christin Brorsson
Department of Chemistry and Cavendish Laboratory, University of Cambridge
The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment.
Article
[162 - 2.520]
Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence
Danielle M. Corgliano, Heike Hofstetter and Oliver Hofstetter
Northern Illinois University, DeKalb, IL, USA
Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen
Article
[152 - 1.140]
Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar
Ganske F, Dell EJ, Larson B
BMG LABTECH 2008
Far-red competitive FP immunoassay for ATP depletion measurements
Article
[158 - 6.170]
Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software
Ganske F, Dell EJ
BMG LABTECH 2007
The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window.
Article
[135 - 2.430]
Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader
Poon S, Rivers RC
Department of Chemistry, University of Cambridge, UK.
Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons.
Article
[133 - 5.110]
Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader
Mack L, Barton A
BMG LABTECH 2005
Detection and quantification of rheumatoid factors (immunoglobulins Igs)
Article
[128 - 2.410]
High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader
Aucamp J, Cosme AM, Lye GJ, Dalby PA
BMG LABTECH 2005
Unfolding transitions of proteins are observed by measuring tryptophan fluorescence
Article
[103 - 2.018]
Molecular Probes PicoGreen assay performed on BMG LABTECH POLARstar OPTIMA microplate reader
Angersbach S, Earp R.
BMG LABTECH
A standard DNA quantification assay using fluorescence
Article
[110 - 4.012]
Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy
Earp RL, Cheek J
BMG LABTECH
The ATP detection kit is linear through-out kit range
Article
[116 - 6.002]
DNA Quantification (Absorbance Mode)
BMG LABTECH, Germany
The most common method for quantifying DNA samples is by conventional absorbance measurements.
Article
[113 - 4.007]
The use of an ATP bioluminescence assay to quantify cell cytotoxicity
Hipler B.
BMG LABTECH
Classical ATP cell cytotoxicity assay based on firefly activity
Article
High Throughput Screening
GPCR (G-Protein Coupled Receptors)
[221 - 4.370]
Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega
K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt
BMG LABTECH 2012
Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production.
Article
[210 - 7.140]
An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on BMG LABTECH's POLARstar Omega
J.D. Brown, R. Mould and C.J. Langmead
BMG LABTECH 2011
POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay.
Article
[209 - 3.200]
GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay
Jacquemart L, Dell EJ
BMG LABTECH 2010
Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection
Article
[206 - 2.680]
Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader
Mark Koeppel, Invitrogen, Madison Wisconsin, USA; EJ Dell, BMG LABTECH, NC, USA
BMG LABTECH 2010
Two fluorescent cell-based assays from Invitrogen performed on the PHERAstar FS with direct bottom reading and high resolution well scanning
Article
[194 - 8.150]
FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction
Hutsell SQ, Kimple AJ, Siderovski DP
BMG LABTECH 2009
CFP-YFP FRET based assay utilized to screen for potential inhibitors
Article
[196 - 2.620]
Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation
Muller RE, Siderovski DP, Kimple AJ, Dell EJ
BMG LABTECH 2009
Alternative, non-radiological method to assess heterotrimeric G protein activation
Article
[193 - 3.190]
Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
Bernd Laffert and Franka Ganske
BMG LABTECH
HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany.
Article
[157 - 2.500]
Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest
Liao J, Dell E.J.
ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007
NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist
Article
[143 - 4.230]
Study of GPCR pharmacology using the DiscoveRx HitHunter
Rainard JM, Mireylees SE, Darlison MG
BMG LABTECH 2006
Luminescence-based assay to detect low levels of cAMP
Article
[142 - 1.030]
HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar
Kauffman L, De La Llera, Angersbach S, Orban M
BMG LABTECH 2006
A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels
Article
[134 - 4.200]
New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar
Kauffman L, De La Llera S, Orban M
BMG LABTECH 2006
Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format
Article
[107 - 2.013]
Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader
Hoefgen B, Kassack MU.
Calcium measurements of Oregon Green loaded HEK293 cells
Application Note PDF
Kinases, Cytochrome P450 and Oxygenase
[202 - 4.340]
Promega's ADP-Glo(TM) Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega
Ganske, F, Dell, EJ, Shultz S
BMG LABTECH 2009
A homogeneous luminescent assay from Promega to detect ADP
Article
[203 - 2.650]
Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
George Cathcart, Brendan Gilmore, Brian Walker.
BMG LABTECH 2009
Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors
Article
[201 - 2.660]
The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers
Franka Ganske, BMG LABTECH, Offenburg, Germany; EJ Dell, BMG LABTECH, NC, USA / Meera Kumar, BellBrook Labs, Madison, USA
BMG LABTECH 2009
The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay
Article
[185 - 6.250]
Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA
Franka Ganske
BMG LABTECH 2009
Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk).
Article
[183 - 1.180]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP
Article
[182 - 1.170]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's PHERAstar Plus HTS Microplate Reader
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP
Article
[186 - 7.120]
AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega
Franka Ganske
BMG LABTECH 2008
In this application note AlphaScreen® instrument settings and sensitivity as well as Z´value of a tyrosine kinase assay are presented. The measurements were done using Omega instruments.
Article
[179 - 2.580]
Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA
Bonnett, S.A.F., Leah, R., and Maltby, E
BMG LABTECH 2008
Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes
Article
[167 - 6.180]
Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA
Papadia S, Léveillé F and Hardingham GE
BMG LABTECH 2008
The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm.
Article
[152 - 1.140]
Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar
Ganske F, Dell EJ, Larson B
BMG LABTECH 2008
Far-red competitive FP immunoassay for ATP depletion measurements
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[151 - 4.250]
Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA
Cali J, Dell EJ
Promega Corporation, BMG LABTECH, Germany
Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase
Article
[153 - 7.110]
Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode
Ganske F and Orban M.
BMG LABTECH, Germany, 2007
Laser-based AlphaScreen® is demonstrated in this application note. PHERAstar instrument settings are given and a titration curve of biotinylated and phosphorylated polypeptide (biot-LCK-P) is presented.
Article
[141 - 2.450]
ADP HunterTM Assay for HTS of Kinase Inhibitors using the PHERAstar
Kauffman L, Hguyen U, Angersbach S, Orban M
BMG LABTECH 2006
The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases
Article
[132 - 2.400]
Development of a post-reaction derivatisation and Fluorescence assay for 2-OG dependent dioxygenases
McNeill LA, Hewitson KS, Schonfeld CJ, Bethge L.
BMG LABTECH 2004
NOVOstar was used both as incubator and reader for measuring the activity of dioxygenases
Article
[131 - 4.180]
Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader
Paine A, Walker L.
BMG LABTECH 2004
Screening of target enzymes without need of specific antibodies or radioactive beads
Article
[127 - 3.127]
A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar
Rossant CJ, Brown JP, Marshall ML.
BMG LABTECH 2004
Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition
Article
Second Messenger Signalling; cAMP
[221 - 4.370]
Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega
K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt
BMG LABTECH 2012
Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production.
Article
[209 - 3.200]
GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay
Jacquemart L, Dell EJ
BMG LABTECH 2010
Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[134 - 4.200]
New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar
Kauffman L, De La Llera S, Orban M
BMG LABTECH 2006
Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format
Article
Second messenger Signalling; Calcium
[216 - 4.350]
Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega
Bodo Ortmann, Silke Valentin, Kristin Atze, Nadine Breuer, Steffi Franke, Mathias Kühn and Leon de Bruin
BMG LABTECH Application Note 2011
Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements
Article
[206 - 2.680]
Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader
Mark Koeppel, Invitrogen, Madison Wisconsin, USA; EJ Dell, BMG LABTECH, NC, USA
BMG LABTECH 2010
Two fluorescent cell-based assays from Invitrogen performed on the PHERAstar FS with direct bottom reading and high resolution well scanning
Article
[157 - 2.500]
Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest
Liao J, Dell E.J.
ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007
NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist
Article
[104 - 2.025]
Intracellular Calcium Assay performed on NOVOstar Microplate Reader
Heding A.
BMG LABTECH
Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format
Article
[107 - 2.013]
Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader
Hoefgen B, Kassack MU.
Calcium measurements of Oregon Green loaded HEK293 cells
Application Note PDF
Second messenger Signalling; Inositol Phosphate
[209 - 3.200]
GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay
Jacquemart L, Dell EJ
BMG LABTECH 2010
Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection
Article
[142 - 1.030]
HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar
Kauffman L, De La Llera, Angersbach S, Orban M
BMG LABTECH 2006
A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels
Article
[137 - 3.130]
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
Orban M, Degorce F, Tardieu JL
BMG LABTECH 2005
Comprehensive overview of GPCRs in response to known agonists
Article
[127 - 3.127]
A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar
Rossant CJ, Brown JP, Marshall ML.
BMG LABTECH 2004
Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition
Article
Ion Channels (Electrophysiology)
[175 - 2.560]
Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader
Heather Mortiboys and Oliver Bandmann
BMG LABTECH 2008
The membrane potential was measured in fibroblast cells in 96-well and 384-well format
Article
[173 - 1.150]
Performance of the PredictorTM hERG Fluorescence Polarization Assay Kit using the PHERAstar
Dell EJ
BMG LABTECH 2008
Nonradioactive patch-clamp alternative to test drug candidates for blockage of the hERG channel
Article
[123 - 8.110]
Ion channel assay development using Invitrogen's FRET-based voltage sensor probes
Hoffman RL, Duff S, Qadir N.
Invitrogen Corporation, Madison, WI 53719, USA
POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays.
Article
HTRF (Homogeneous Time-Resolved Fluorescence) Assays
[193 - 3.190]
Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
Bernd Laffert and Franka Ganske
BMG LABTECH
HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany.
Article
[177 - 3.170]
HTRF®IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus
Ganske F, Jacquemart L, Jean A, Lebreton ML, Tardieu JL
BMG LABTECH 2008
An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[154 - 3.160]
Cisbio´s HTRF® cortisol assay performed on the PHERAstar
Ganske F, Tardieu JL and Degorce F.
BMG LABTECH, Germany and Cisbio International, France
Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined
Article
[137 - 3.130]
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
Orban M, Degorce F, Tardieu JL
BMG LABTECH 2005
Comprehensive overview of GPCRs in response to known agonists
Article
Solubility Measured with Nephelometry
[174 - 5.120]
Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar
U.-C. Hipler
BMG LABTECH 2008
Laser nephelometry used for fungal growth determination in presence of antifungal agents
Article
[117 - 5.101]
A fully automated solubility screen in 384-well plate format using nephelometry
Green C, McKee S, Saunders K.
BMG LABTECH 2003
Kinetic solubility determination of 24 compounds in 75 min
Article
Single Nucleotide Polymorphisms (SNP Genotyping)
[187 - 2.640]
Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader
Hamilton A, Liang R, Whittle B
BMG LABTECH 2010
Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore
Article
[160 - 8.130]
High Speed FRET based SNP Genotyping Measurement on the PHERAstar
Robinson P and Ganske F
BMG LABTECH 2007
KASPar™ System from KBioScience optimally measured with dual emission optical modules
Article
[129 - 8.120]
Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping
Robinson P.
BMG LABTECH 2004
SNP Genotyping using Fam, Hex and Rox as labels
Article
Reporter Gene Assays
[221 - 4.370]
Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega
K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt
BMG LABTECH 2012
Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production.
Article
[199 - 4.330]
Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA
Eboigbodin KE and Robinson GK
BMG LABTECH 2009
Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth.
Article
[184 - 6.240]
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega
Mutch BE, Willetts K, Timms E, Foxwell BMJ
BMG LABTECH 2008
The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR).
Article
[172 - 4.300]
Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon
Gretton S, Harris M
BMG LABTECH 2008
DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA
Article
[165 - 4.280]
Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers
Dell EJ
BMG LABTECH 2007
DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors
Article
Protein-Protein/Protein-DNA interactions
[213 - 2.720]
The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH
Miriam Uppill, Antony Sheehan and Ron Osmond
BMG LABTECH 2011
Fluorescent based ELISA with only one wash step measured on the POLARstar Omega
Article
[190 - 7.130]
Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus
Franka Ganske and Keith Ansell
BMG LABTECH 2009
Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented.
Article
[180 - 1.160]
High-throughput protein-DNA affinity measurements using fluorescence anisotropy
Veprintsev DB
BMG LABTECH 2008
The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied.
Article
Microbial Growth / Cellular Analysis
Cell Growth Kinetics
[155 - 6.150]
Growth of Neisseria meningitidis in the FLUOstar OPTIMA with Microprocessor Controlled Gas Vent
Cutter KL
BMG LABTECH Application Notes 2007
This study highlights the advantage of an automated controlled gas system over a carbon dioxide incubator.
Article
[217 - 6.320]
Growth of Campylobacter using BMG LABTECH's FLUOstar Omega equipped with ACU
Haigh RD and Ketley JM
BMG LABTECH 2011
Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU
Article
[207 - 6.300]
Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay
Hynes J, Carey C, Wark C
BMG LABTECH 2010
MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel.
Article
[199 - 4.330]
Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA
Eboigbodin KE and Robinson GK
BMG LABTECH 2009
Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth.
Article
[191 - 6.270]
High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure on the FLUOstar OPTIMA
S. Lucas Black, F. Bruce Ward and Rosalind J. Allen
BMG LABTECH 2009
Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity.
Article
[189 - 6.260]
High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega
Gordon Ramage, Ryan Quinn and Steven Milligan
BMG LABTECH 2009
The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic.
Article
[174 - 5.120]
Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar
U.-C. Hipler
BMG LABTECH 2008
Laser nephelometry used for fungal growth determination in presence of antifungal agents
Article
[140 - 6.130]
High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA
Webber MA, Piddock LJ
BMG LABTECH 2006
The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium.
Article
[125 - 5.106]
Monitoring of Microbial Growth Curves by Laser Nephelometry
Angersbach S, Brand S, Hipler B, Rückert C.
BMG LABTECH 2004
Correlation between nephelometry and absorbance in microbial growth monitoring
Article
Detection of Cell Contaminations and Mycoplasma
[130 - 4.170]
Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader
Cox A, Pitt A.
BMG LABTECH 2004
Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay
Article
[114 - 5.005]
Nephelometric Test for Determination of Colostrum Quality
AgResearch, BMG LABTECH Australia
Quantification of IgG1 in milk and colostrum
Article
Cell Viability Assays
[218 - 4.360]
BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay
Andrew Knight, Chris Hughes and Matthew Tate. Gentronix Limited, Manchester, UK.
BMG LABTECH 2011
A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress.
Article
[214 - 2.730]a
Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability
Emily Peak, Iain W. Chalmers and Karl F. Hoffmann
BMG LABTECH 2011
Dual chromatic bottom reading fluorescence assay to determine schistosoma viability
Article
[207 - 6.300]
Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay
Hynes J, Carey C, Wark C
BMG LABTECH 2010
MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel.
Article
[195 - 2.610]
alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA
Markaki AE
BMG LABTECH 2009
Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay
Article
[159 - 2.510]
High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA
Chitolie MS & Toescu EC
BMG LABTECH 208
Cell viability of cerebellar granule cells were measured utilizing propidium iodide
Article
[171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
Rebecca Foster and Kyla Grimshaw
BMG LABTECH 2008
Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay.
Article
[139 - 4.220]
Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
Worzella T, Larson B
BMG LABTECH 2006
Multiplexing reporter gene activity (EnduRen™ or ViviRen™) and cell viability (CellTiter-Glo®) in a single sample well
Article
[138 - 2.440]
Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Worzella T, Larson B
BMG LABTECH 2006
Correlated measurement of an apoptosis marker and cell viability
Article
Cell Cytotoxicity Assays
[218 - 4.360]
BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay
Andrew Knight, Chris Hughes and Matthew Tate. Gentronix Limited, Manchester, UK.
BMG LABTECH 2011
A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress.
Article
[207 - 6.300]
Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay
Hynes J, Carey C, Wark C
BMG LABTECH 2010
MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel.
Article
[192 - 3.180]
A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay performed on the PHERAstar
Patterson NM, Cunningham T, Jiang X, Dell EJ, Ganske F, Shapiro DJ
BMG LABTECH 2009
Estrogen reduces cell death induced by NK92 natural killer cells
Article
[113 - 4.007]
The use of an ATP bioluminescence assay to quantify cell cytotoxicity
Hipler B.
BMG LABTECH
Classical ATP cell cytotoxicity assay based on firefly activity
Article
GFP Expression
[144 - 2.460]
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
Hart CA , Clarke NW, Brown MD
Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system
Article
[119 - 1.101]
Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence
Knight AW
BMG LABTECH
The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay
Article
Tight Junctions
[146 - 2.470]
Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans
Balda MS and Matter K
BMG LABTECH 2007
A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA
Article
Macrophages
[188 - 2.600]
A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA
Sanders, D.A. and Charnock-Jones, D.S.
BMG LABTECH 2009
A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated.
Article
[184 - 6.240]
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega
Mutch BE, Willetts K, Timms E, Foxwell BMJ
BMG LABTECH 2008
The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR).
Article
[156 - 2.490]
Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA
Taylor A, Donelly L.
Thoracic Medicine, National Heart and Lung Institute
Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies
Article
False Positives
[124 - 1.020]
Identifcation of false positives in an FP Screen
Saxty B
BMG LABTECH 2004
Identification of interfering compounds and non specific inhibitors
Article
pH determination
[219 - 3.220]
Assessment of Extracellular Acidification using a Fluorescence-Based Assay
Hynes J, Carey C, Kriwan S, Wark C
BMG LABTECH 2011
Extracellular pH measurement in TRF mode using Luxcel chemistry
Article
Food Chemistry
Antioxidant Determination (ORAC and ABEL®)
[197 - 2.630]
ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader
Franka Ganske und Marjan Orban
BMG LABTECH 2009
The antioxidant capacity of fruit juices is determined in 96-well and 384-well format
Article
[148 - 2.480]
ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity
Ganske F, Dell EJ
BMG LABTECH
A standard assay to determine antioxidant capacity using Trolox® as reference substance
Article
[108 - 4.009]
Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants
Hipler B, Knight J.
Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk
Article
Reactive Oxygen species (ROS) and (Chemiluminescence)
[112 - 4.103]
Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood
Lateef S, Ingram PR, Spickett CM.
Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl)
Article
[111 - 4.006]
Chemiluminescence measurement of the generation of reactive oxygen species
Hipler B, Hipler UC.
Very low ROS concentrations in Candida species detected
Article
[106 - 4.002]
Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans
Hipler UC, Schröter C, Wollina U, Hipler B.
The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader
Article
[105 - 2.008]
Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus
Hipler UC, Wollina U, Denning D, Hipler B.
The intracellular ROS formation is measured using 2',7'-dichlorfluorescein  diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2)
Application Note PDF
Oxygen Sensing
[207 - 6.300]
Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay
Hynes J, Carey C, Wark C
BMG LABTECH 2010
MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel.
Article
Additional Assays
BRET (Bioluminescence resonance energy transfer) assays
[120 - 9.110]
Simultaneous dual emission detection for fast kinetic BRET assays
Orban M.
BMG LABTECH 2003
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
Article
[118 - 9.100]
BRET assays performed on the FLUOstar OPTIMA
Orban M.
BMG LABTECH 2002
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
Application Note PDF
FRET (Fluorescence Resonance Energy Transfer) assays
[194 - 8.150]
FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction
Hutsell SQ, Kimple AJ, Siderovski DP
BMG LABTECH 2009
CFP-YFP FRET based assay utilized to screen for potential inhibitors
Article
[163 - 8.140]
SUMO FRET-based assays performed on BMG LABTECH's NOVOstar
Martin SF
Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar
Article
[122 - 8.100]
Use of CyDye Fluors for Improved FRET Protease Assays on BMG POLARstar Fluorescence Microplate Reader
George J, Norey C.
GE Healthcare
CyDye™ labeled peptides are used as substrates in protease assays
Article
Migration Assay
[204 - 2.670]
Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega
Iman van den Bout
BMG LABTECH 2009
Real-time monitoring of cell migration on different ECM components
Article
[144 - 2.460]
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
Hart CA , Clarke NW, Brown MD
Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system
Article
ADME (Absorption, Distribution, Metabolism and Elimination)
[151 - 4.250]
Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA
Cali J, Dell EJ
Promega Corporation, BMG LABTECH, Germany
Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase
Article
Hormone Signalling
[154 - 3.160]
Cisbio´s HTRF® cortisol assay performed on the PHERAstar
Ganske F, Tardieu JL and Degorce F.
BMG LABTECH, Germany and Cisbio International, France
Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined
Article
Other
[178 - 6.230]
Reaction optimization by parallel kinetic studies with the FLUOstar Omega
Redman JE
BMG LABTECH 2008
Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel.
Article
[145 - 6.140]
Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader
Wiles M.
Plasso Technology Ltd., Sheffield, UK
A detection system to identify and characterise heparin binding proteins is introduced.
Article
[147 - 4.240]
LUMIstar in the production and quality control for ROTAS on-site test reagents
Dix A
Cybersense Biosystems Ltd, Oxon, UK
BMG LABTECH microplate reader used in the quality control of reagent manufacturing
Article
[136 - 4.210]
LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA
Perfect E, Hickey P.
BMG LABTECH 2004
Measurement of certified calibration reference materials in comparison to biological controls
Article
Membranes
[208 - 2.690]
A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition
Melchior DL, Sharom FJ, Dell EJ
BMG LABTECH 2010
The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter
Article
[205 - 1.190]
Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
Kushnareva Y, Dell EJ
BMG LABTECH 2009
DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes
Article
Aggregation
[215 - 6.310]
Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader
Vaiyapuri S and Gibbins JM
BMG LABTECH 2011
Platelet aggregation induced by addition of agonist measured on the NOVOstar in absorbance mode.
Article
[212 - 2.710]
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
Alison Turner UCB
BMG LABTECH 2011
Thioflavin T aggregation assay to determine antibody stability
Article


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