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Application Notes sorted by Assays

Molecular Biology

Measurement of DNA, ATP, Protein

  Dell EJ [170 - 6.120]
Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
 BMG LABTECH 2008
 Article
  Dell EJ and Ganske F [169 - 4.290]
Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation
 BMG LABTECH 2008
 Article
  Ganske F [168 - 6.190]
Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
 BMG LABTECH 2008
 Article
  Tracey Madgett, University of the West of England, Bristol [166 - 2.540]
Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
 BMG LABTECH 2008
 Article
  Sarah Shammas and Ann-Christin Brorsson [164 - 2.530]
Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation
 Department of Chemistry and Cavendish Laboratory, University of Cambridge
 Article
  Danielle M. Corgliano, Heike Hofstetter and Oliver Hofstetter [162 - 2.520]
Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence
 Northern Illinois University, DeKalb, IL, USA
 Article
  Ganske F, Dell EJ [152 - 1.140]
TranscreenerTM KINASE Fluorescence Polarization Assay Performed on the PHERAstar
 BMG LABTECH 2007
 Article
  Ganske F, Dell EJ [158 - 6.170]
Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software
 BMG LABTECH 2007
 Article
  Poon S, Rivers RC [135 - 2.430]
Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader
 Department of Chemistry, University of Cambridge, UK.
 Article
  Mack L, Barton A [133 - 5.110]
Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader
 BMG LABTECH 2005
 Article
  Aucamp J, Cosme AM, Lye GJ, Dalby PA [128 - 2.410]
High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader
 BMG LABTECH 2005
 Article
  Angersbach S, Earp R. [103 - 2.018]
Molecular Probes PicoGreen assay performed on BMG LABTECH POLARstar OPTIMA microplate reader
 BMG LABTECH
 Article
  Earp RL, Cheek J [110 - 4.012]
Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy
 BMG LABTECH
 Article
  BMG LABTECH, Germany [116 - 6.002]
DNA Quantification (Absorbance Mode)
The most common method for quantifying DNA samples is by conventional absorbance measurements.
 Article
  Hipler B. [113 - 4.007]
The use of an ATP bioluminescence assay to quantify cell cytotoxicity
 BMG LABTECH
 Article

High Throughput Screening

GPCR (G-Protein Coupled Receptors)

  Liao J, Dell E.J. [157 - 2.500]
Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest
 ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007
 Article
  Rainard JM, Mireylees SE, Darlison MG [143 - 4.230]
Study of GPCR pharmacology using the DiscoveRx HitHunter
 BMG LABTECH 2006
 Article
  Kauffman L, De La Llera, Angersbach S, Orban M [142 - 1.030]
HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar
 BMG LABTECH 2006
 Article
  Kauffman L, De La Llera S, Orban M [134 - 4.200]
New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar
 BMG LABTECH 2006
 Article
  Hoefgen B, Kassack MU. [107 - 2.013]
Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader
 Application Note Filesize: (591 kb)

Kinases, Cytochrome P450 and Oxygenase

  Papadia S, Léveillé F and Hardingham GE [167 - 6.180]
Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA
 BMG LABTECH 2008
 Article
  Ganske F, Dell EJ [152 - 1.140]
TranscreenerTM KINASE Fluorescence Polarization Assay Performed on the PHERAstar
 BMG LABTECH 2007
 Article
  Orban M, Angersbach S, Hoffman R, Buros M. [126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
 BMG LABTECH 2005
 Application Note Filesize: (263 kb)
  Cali J, Dell EJ [151 - 4.250]
Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA
 Promega Corporation, BMG LABTECH, Germany
 Article
  Ganske F and Orban M. [153 - 7.110]
Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode
 BMG LABTECH, Germany, 2007
 Article
  Hoffman R, Buros M, Kupcho K, Dell EJ [150 - 3.150]
Invitrogen's LanthascreenTM TR-FRET Tyrosine Kinase Assay Using the PHERAstar
 BMG LABTECH 2007
 Article
  Hoffman R, Buros M, Kupcho K, Dell EJ [149 - 3.140]
Invitrogen's LanthascreenTM TR-FRET Protein Kinase C Assay Using the PHERAstar
 BMG LABTECH 2006
 Article
  Kauffman L, Hguyen U, Angersbach S, Orban M [141 - 2.450]
ADP HunterTM Assay for HTS of Kinase Inhibitors using the PHERAstar
 BMG LABTECH 2006
 Article
  McNeill LA, Hewitson KS, Schonfeld CJ, Bethge L. [132 - 2.400]
Development of a post-reaction derivatisation and Fluorescence assay for 2-OG dependent dioxygenases
 BMG LABTECH 2004
 Article
  Paine A, Walker L. [131 - 4.180]
Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader
 BMG LABTECH 2004
 Article
  Rossant CJ, Brown JP, Marshall ML. [127 - 3.127]
A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar
 BMG LABTECH 2004
 Article

Second Messenger Signalling; cAMP

  Orban M, Angersbach S, Hoffman R, Buros M. [126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
 BMG LABTECH 2005
 Application Note Filesize: (263 kb)
  Kauffman L, De La Llera S, Orban M [134 - 4.200]
New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar
 BMG LABTECH 2006
 Article

Second messenger Signalling; Calcium

  Liao J, Dell E.J. [157 - 2.500]
Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest
 ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007
 Article
  Heding A. [104 - 2.025]
Intracellular Calcium Assay performed on NOVOstar Microplate Reader
 BMG LABTECH
 Article
  Hoefgen B, Kassack MU. [107 - 2.013]
Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader
 Application Note Filesize: (591 kb)

Second messenger Signalling; Inositol Phosphate

  Kauffman L, De La Llera, Angersbach S, Orban M [142 - 1.030]
HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar
 BMG LABTECH 2006
 Article
  Orban M, Degorce F, Tardieu JL [137 - 3.130]
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
 BMG LABTECH 2005
 Article
  Rossant CJ, Brown JP, Marshall ML. [127 - 3.127]
A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar
 BMG LABTECH 2004
 Article

Ion Channels (Electrophysiology)

  Dell EJ [173 - 1.150]
Performance of the PredictorTM hERG Fluorescence Polarization Assay Kit using the PHERAstar
 BMG LABTECH 2008
 Article
  Hoffman RL, Duff S, Qadir N. [123 - 8.110]
Ion channel assay development using Invitrogen's FRET-based voltage sensor probes
 Invitrogen Corporation, Madison, WI 53719, USA
 Article

HTRF (Homogeneous Time-Resolved Fluorescence) Assays

  Orban M, Angersbach S, Hoffman R, Buros M. [126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
 BMG LABTECH 2005
 Application Note Filesize: (263 kb)
  Ganske F, Tardieu JL and Degorce F. [154 - 3.160]
Cisbio´s HTRF® cortisol assay performed on the PHERAstar
 BMG LABTECH, Germany and Cisbio International, France
 Article
  Orban M, Degorce F, Tardieu JL [137 - 3.130]
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
 BMG LABTECH 2005
 Article

Solubility Measured with Nephelometry

  Green C, McKee S, Saunders K. [117 - 5.101]
A fully automated solubility screen in 384-well plate format using nephelometry
 BMG LABTECH 2003
 Article

Single Nucleotide Polymorphisms (SNP Genotyping)

  Robinson P and Ganske F [160 - 8.130]
High Speed FRET based SNP Genotyping Measurement on the PHERAstar
 BMG LABTECH 2007
 Article
  Robinson P. [129 - 8.120]
Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping
 BMG LABTECH 2004
 Article

Reporter Gene Assays

  Dell EJ [165 - 4.280]
Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers
 BMG LABTECH 2007
 Article

Microbial Growth / Cellular Analysis

Cell Growth Kinetics

  Webber MA, Piddock LJ [140 - 6.130]
High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA
 BMG LABTECH 2006
 Article
  Angersbach S, Brand S, Hipler B, Rückert C. [125 - 5.106]
Monitoring of Microbial Growth Curves by Laser Nephelometry
 BMG LABTECH 2004
 Article

Detection of Cell Contaminations and Mycoplasma

  Cox A, Pitt A. [130 - 4.170]
Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader
 BMG LABTECH 2004
 Article
  AgResearch, BMG LABTECH Australia [114 - 5.005]
Nephelometric Test for Determination of Colostrum Quality
 Article

Cell Viability Assays

  Chitolie MS & Toescu EC [159 - 2.510]
High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA
 BMG LABTECH 208
 Article
  Rebecca Foster and Kyla Grimshaw [171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
 BMG LABTECH 2008
 Article
  Worzella T, Larson B [139 - 4.220]
Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
 BMG LABTECH 2006
 Article
  Worzella T, Larson B [138 - 2.440]
Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
 BMG LABTECH 2006
 Article

Cell Cytotoxicity Assays

  Hipler B. [113 - 4.007]
The use of an ATP bioluminescence assay to quantify cell cytotoxicity
 BMG LABTECH
 Article

GFP Expression

  Hart CA , Clarke NW, Brown MD [144 - 2.460]
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
 Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
 Article
  Knight AW [119 - 1.101]
Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence
 BMG LABTECH
 Article

Tight Junctions

  Balda MS and Matter K [146 - 2.470]
Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans
 BMG LABTECH 2007
 Article

Macrophages

  Taylor A, Donelly L. [156 - 2.490]
Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA
 Thoracic Medicine, National Heart and Lung Institute
 Article

False Positives

  Saxty B [124 - 1.020]
Identifcation of false positives in an FP Screen
 BMG LABTECH 2004
 Article

Food Chemistry

Antioxidant Determination (ORAC and ABEL®)

  Ganske F, Dell EJ [148 - 2.480]
ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity
 BMG LABTECH
 Article
  Hipler B, Knight J. [108 - 4.009]
Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants
 Article

Reactive Oxygen species (ROS) and (Chemiluminescence)

  Lateef S, Ingram PR, Spickett CM. [112 - 4.103]
Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood
 Article
  Hipler B, Hipler UC. [111 - 4.006]
Chemiluminescence measurement of the generation of reactive oxygen species
 Article
  Hipler UC, Schröter C, Wollina U, Hipler B. [106 - 4.002]
Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans
 Article
  Hipler UC, Wollina U, Denning D, Hipler B. [105 - 2.008]
Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus
 Application Note Filesize: (235 kb)

Additional Assays

BRET (Bioluminescence resonance energy transfer) assays

  Orban M. [120 - 9.110]
Simultaneous dual emission detection for fast kinetic BRET assays
 BMG LABTECH 2003
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
 Article
  Orban M. [118 - 9.100]
BRET assays performed on the FLUOstar OPTIMA
 BMG LABTECH 2002
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
 Application Note Filesize: (914 kb)

FRET (Fluorescence Resonance Energy Transfer) assays

  Martin SF [163 - 8.140]
SUMO FRET-based assays performed on BMG LABTECH's NOVOstar
 Article
  George J, Norey C. [122 - 8.100]
Use of CyDye Fluors for Improved FRET Protease Assays on BMG POLARstar Fluorescence Microplate Reader
 GE Healthcare
 Article
 Link

Migration Assay

  Hart CA , Clarke NW, Brown MD [144 - 2.460]
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
 Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
 Article

ADME (Absorption, Distribution, Metabolism and Elimination)

  Cali J, Dell EJ [151 - 4.250]
Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA
 Promega Corporation, BMG LABTECH, Germany
 Article

Hormone Signalling

  Ganske F, Tardieu JL and Degorce F. [154 - 3.160]
Cisbio´s HTRF® cortisol assay performed on the PHERAstar
 BMG LABTECH, Germany and Cisbio International, France
 Article

Other

  Wiles M. [145 - 6.140]
Plasso EpranEx" ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader
 Plasso Technology Ltd., Sheffield, UK
 Article
  Dix A [147 - 4.240]
LUMIstar in the production and quality control for ROTAS on-site test reagents
 Cybersense Biosystems Ltd, Oxon, UK
 Article
  Perfect E, Hickey P. [136 - 4.210]
LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA
 BMG LABTECH 2004
 Article


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