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Absorbance
[207 - 6.300]
Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay
Hynes J, Carey C, Wark C
BMG LABTECH 2010
Redlight"from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel.
Article
[199 - 4.330]
Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA
Eboigbodin KE and Robinson GK
BMG LABTECH 2009
Article
[185 - 6.250]
Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA
Franka Ganske
BMG LABTECH 2009
Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk).
Article
[191 - 6.270]
High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure on the FLUOstar OPTIMA
S. Lucas Black, F. Bruce Ward and Rosalind J. Allen
BMG LABTECH 2009
Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity.
Article
[189 - 6.260]
High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega
Gordon Ramage, Ryan Quinn and Steven Milligan
BMG LABTECH 2009
The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic.
Article
[184 - 6.240]
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega
Mutch BE, Willetts K, Timms E, Foxwell BMJ
BMG LABTECH 2008
The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR).
Article
[178 - 6.230]
Reaction optimization by parallel kinetic studies with the FLUOstar Omega
Redman JE
BMG LABTECH 2008
Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel.
Article
[170 - 6.210]
Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
Dell EJ
BMG LABTECH 2008
UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm.
Article
[171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
Rebecca Foster and Kyla Grimshaw
BMG LABTECH 2008
Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay.
Article
[169 - 6.200]
Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation
Dell EJ and Ganske F
BMG LABTECH 2008
Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented.
Article
[167 - 6.180]
Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA
Papadia S, Léveillé F and Hardingham GE
BMG LABTECH 2008
The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm.
Article
[168 - 6.190]
Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
Ganske F
BMG LABTECH 2008
DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented.
Article
[158 - 6.170]
Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software
Ganske F, Dell EJ
BMG LABTECH 2007
The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window.
Article
[145 - 6.140]
Plasso EpranEx" ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader
Wiles M.
Plasso Technology Ltd., Sheffield, UK
Article
[140 - 6.130]
High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA
Webber MA, Piddock LJ
BMG LABTECH 2006
The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium.
Article
[135 - 2.430]
Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader
Poon S, Rivers RC
Department of Chemistry, University of Cambridge, UK.
Article
[116 - 6.002]
DNA Quantification (Absorbance Mode)
BMG LABTECH, Germany
The most common method for quantifying DNA samples is by conventional absorbance measurements.
Article

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